Araştırma Çıktıları

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Author: search.filters.author.Acar, NurSubject: search.filters.subject.diabetic retinopathy

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    Protective effect of pomegranate juice on retinal oxidative stress in streptozotocin-induced diabetic rats
    (IJO PRESS, 2017-01-01) Tugcu, Betul; Nacaroglu, Senay Asik; Gedikbasi, Asuman; Uhri, Mehmet; Acar, Nur; Ozdemir, Hakan
    AIM: To investigate the effect of pomegranate juice (PJ) intake on overall oxidation status in retinas of diabetic rats. METHODS: Twenty-seven rats were divided into four groups as control (CO), diabetic (DM), control treated with PJ (CO-PJ), and diabetic treated with PJ (DM-PJ). The retina tissues were used to determine 8-hydroxy2 `-deoxyguanosine (8OHdG), malondialdehyde (MDA), reduced glutathione (GSH) levels, and the enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). RESULTS: The levels of 8OHdG and MDA were significantly increased in the retina of DM group compared to CO group (P= 0.001, P< 0.001 respectively). Both 8OHdG and MDA levels were decreased in PJ-DM group compared to DM group (P= 0.004, P< 0.001 respectively). The activities of antioxidant enzymes GSH, SOD, and GDH-Px were significantly decreased in the retina of DM group compared to CO group (P= 0.01). GSH and GSH-Px activities were higher in PJ-DM group compared with DM group (P= 0.010, P= 0.042, respectively) but SOD activity was not statistically different (P= 0.938). CONCLUSION: PJ intake is found to be effective in decreasing oxidative end products, and in increasing the activities of antioxidant enzymes in diabetic retinas of rats, which suggests it may be effective against oxidative stress in diabetic retinas.
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    The assessment of autofluorescence of the crystalline lens in diabetic patients and healthy controls: can it be used as a screening test?
    (DOVE MEDICAL PRESS LTD, 2018-01-01) Pehlivanoglu, Seren; Acar, Nur; Albayrak, Sinan; Karakaya, Muharrem; Ofluoglu, Ali
    Background: Our purpose was to demonstrate if measuring lens autofluorescence (AF) with a scanning confocal biomicroscope may be used to identify subjects with undiagnosed type II diabetes mellitus (DM), and hence, for it to be used as a marker for the severity of diabetic retinopathy in diabetic patients. Patients and methods: In this cross-sectional, comparative study, lens AF was measured with scanning confocal lens fluorescence biomicroscope in diabetic and healthy groups. Full ophthalmological examination was performed. Blood tests of fasting plasma glucose, and glycosylated hemoglobin were also analyzed. The correlation between lens AF results and blood tests was evaluated in both groups. The cutoff value for the diagnosis of DM using lens AF was investigated. Results: The study included 191 subjects with a mean age of 52.09 +/- 6.75 years. One hundred and seven (56.0\%) subjects were female, and 84 (44.0\%) were male. Eighty-two (42.9\%) patients had type II DM, and 109 (57.1\%) subjects self-reported as normal. The fluorescence ratio (FR) values ranged from 0.09 to 0.46 (0.23 +/- 0.06) in the total group. Mean FR measurements of diabetic subjects were significantly higher (0.27 +/- 0.06) than those without DM (0.20 +/- 0.05), (p=0.001). A statistically significant correlation was found between glycosylated hemoglobin, fasting plasma glucose, and FR. The cutoff point for the FR according to the presence of DM was found to be 0.24 and above (p=0.001), with a sensitivity of 71.95\% and a specificity of 80.73\%. Conclusion: Measuring AF of human lens as an indirect evidence of increased advanced glycaton end products may helpful in detecting impaired glucose metabolism. Our results show highly significant correlation between possibility of DM and FR.