Ligand binding pocket of a novel Allatostatin receptor type C of stick insect, Carausius morosus
Ligand binding pocket of a novel Allatostatin receptor type C of stick insect, Carausius morosus
Dosyalar
Tarih
2017-01-01
Yazarlar
Sahbaz, Burcin Duan
Sezerman, Osman Ugur
Torun, Hamdi
Iyison, Necla Birgul
Süreli Yayın başlığı
Süreli Yayın ISSN
Cilt Başlığı
Yayınevi
NATURE PUBLISHING GROUP
Dergi Adı
SCIENTIFIC REPORTS
Özet
Allatostatins (AST) are neuropeptides with variable function ranging from regulation of developmental processes to the feeding behavior in insects. They exert their effects by binding to cognate GPCRs, called Allatostatin receptors (AlstR), which emerge as promising targets for pesticide design. However, AlstRs are rarely studied. This study is the first reported structural study on AlstR-AST interaction. In this work, the first C type AlstR from the stick insect Carausius morosus (CamAlstR-C) was identified and its interaction with type C AST peptide was shown to be physically consistent with the experimental results. The proposed structure of CamAlstR-C revealed a conserved motif within the third extracellular loop, which, together with the N-terminus is essential for ligand binding. In this work, computational studies were combined with molecular and nano-scale approaches in order to introduce an unknown GPCR-ligand system. Consequently, the data obtained provided a reliable target region for future agonist/inverse agonist studies on AlstRs.