Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species

dc.contributor.authorAkyar, Isin
dc.contributor.authorCavusoglu, Cengiz
dc.contributor.authorAyas, Meltem
dc.contributor.authorSurucuoglu, Suheyla
dc.contributor.authorIlki, Arzu
dc.contributor.authorKaya, Deniz Ece
dc.contributor.authorBesli, Yesim
dc.date.accessioned2023-02-21T12:37:47Z
dc.date.available2023-02-21T12:37:47Z
dc.date.issued2018-01-01
dc.description.abstractBackground/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100\% of MTC and in 91\% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.
dc.description.issue6
dc.description.pages1351-1357
dc.description.volume48
dc.identifier.doi10.3906/sag-1801-198
dc.identifier.urihttps://hdl.handle.net/11443/2295
dc.identifier.urihttp://dx.doi.org/10.3906/sag-1801-198
dc.identifier.wosWOS:000452890300037
dc.publisherTUBITAK SCIENTIFIC \& TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF MEDICAL SCIENCES
dc.subjectMALDI-TOF MS
dc.subjectidentification
dc.subjectMycobacterium
dc.subjectMycobacterium tuberculosis complex
dc.subjectnontuberculous mycobacteria
dc.titleEvaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species
dc.typeArticle

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