Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species
dc.contributor.author | Akyar, Isin | |
dc.contributor.author | Cavusoglu, Cengiz | |
dc.contributor.author | Ayas, Meltem | |
dc.contributor.author | Surucuoglu, Suheyla | |
dc.contributor.author | Ilki, Arzu | |
dc.contributor.author | Kaya, Deniz Ece | |
dc.contributor.author | Besli, Yesim | |
dc.date.accessioned | 2023-02-21T12:37:47Z | |
dc.date.available | 2023-02-21T12:37:47Z | |
dc.date.issued | 2018-01-01 | |
dc.description.abstract | Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100\% of MTC and in 91\% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC. | |
dc.description.issue | 6 | |
dc.description.pages | 1351-1357 | |
dc.description.volume | 48 | |
dc.identifier.doi | 10.3906/sag-1801-198 | |
dc.identifier.uri | https://hdl.handle.net/11443/2295 | |
dc.identifier.uri | http://dx.doi.org/10.3906/sag-1801-198 | |
dc.identifier.wos | WOS:000452890300037 | |
dc.publisher | TUBITAK SCIENTIFIC \& TECHNICAL RESEARCH COUNCIL TURKEY | |
dc.relation.ispartof | TURKISH JOURNAL OF MEDICAL SCIENCES | |
dc.subject | MALDI-TOF MS | |
dc.subject | identification | |
dc.subject | Mycobacterium | |
dc.subject | Mycobacterium tuberculosis complex | |
dc.subject | nontuberculous mycobacteria | |
dc.title | Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species | |
dc.type | Article |
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