Investigation of the Effect of Channel Structure and Flow Rate on On-Chip Bacterial Lysis
Investigation of the Effect of Channel Structure and Flow Rate on On-Chip Bacterial Lysis
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Tarih
2021-01-01
Yazarlar
Dizaji, Araz Norouz
Ozturk, Yasin
Ghorbanpoor, Hamed
Cetak, Ahmet
Akcakoca, Iremnur
Kocagoz, Tanil
Avci, Huseyin
Corrigan, Damion
Guzel, Fatma Dogan
Süreli Yayın başlığı
Süreli Yayın ISSN
Cilt Başlığı
Yayınevi
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Dergi Adı
IEEE TRANSACTIONS ON NANOBIOSCIENCE
Özet
Successful lysis of cells/microorganisms is a key step in the sample preparation in fields like molecular biology, bioengineering, and biomedical engineering. This study therefore aims to investigate the lysis of bacteria on-chip and its dependence on both microfluidic channel structure and flow rate. Effects of temperature on lysis on-chip were also investigated. To perform these investigations, three different microfluidic chips were designed and produced (straight, zigzag and circular configurations), while the length of the channels were kept constant. As an exemplary case, Mycobacterium smegmatis was chosen to represent the acid-fast bacteria. Bacterial suspensions of 1.5 McFarland were injected into the chips at various flow rates (0.6-8 mu l/min) either at room temperature or 50 degrees C. In order to understand the on-chip lysis performance fully, off-chip experiments were carried out at durations which are equal to those bacteria spent in the channel from inlet to the outlet at different flow rates. We also performed COMSOL multiphysics program simulations to evaluate further the effect of the applied parameters. As a result, we found that the structure and the flow rate do not affect lysis over all in all investigated channel types, however on-chip experiments at room temperature produced more effective lysis compared to the on-chip and the off-chip samples performed at higher temperatures. Interestingly on-chip experiments at higher tempratures do not result in effective lysis.
Açıklama
Anahtar kelimeler
Microfluidic chip, thermal lysis, on-chip bacterial lysis, Mycobacterium smegmatis