Tumor necrosis factor-alpha induced caspase-3 activation-related iNOS gene expression in ADP-activated platelets
dc.contributor.author | Cevik, Ozge | |
dc.contributor.author | Adiguzel, Zelal | |
dc.contributor.author | Baykal, Ahmet Tarik | |
dc.contributor.author | Sener, Azize | |
dc.date.accessioned | 2023-02-21T12:36:41Z | |
dc.date.available | 2023-02-21T12:36:41Z | |
dc.date.issued | 2017-01-01 | |
dc.description.abstract | Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-a in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-alpha and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding | |
dc.description.abstract | cGMP, Cyt-c, and Ca2+ levels and NOS activation | |
dc.description.abstract | and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-a and/or 50 mM iNOS specific inhibitor, such as at 1400 W for 1 h at 37 degrees C in the presence of 5 mu M ADP. According to the results, the levels of PS exposure and P-selectin binding were significantly higher in TNF-alpha + ADP platelets, which decreased with the addition of 1400 W. A significant induction in cGMP, Cyt-c, and Ca2+ levels was observed in platelets treated with TNF-alpha + ADP. On the other hand, the upregulation of the apoptotic gene caspase-3 and iNOS expression levels in TNF-alpha-treated and ADP-activated platelets was significantly reversed with the addition of 1400 W. These data demonstrate that TNF-alpha promotes iNOS expression through caspase-3 stimulation in human platelets, and its concomitance leads to the triggering of apoptosis-mediated inflammation upon platelet activation. | |
dc.description.issue | 1 | |
dc.description.pages | 31-40 | |
dc.description.volume | 41 | |
dc.identifier.doi | 10.3906/biy-1509-64 | |
dc.identifier.uri | https://hdl.handle.net/11443/2134 | |
dc.identifier.uri | http://dx.doi.org/10.3906/biy-1509-64 | |
dc.identifier.wos | WOS:000394539800004 | |
dc.publisher | TUBITAK SCIENTIFIC \& TECHNICAL RESEARCH COUNCIL TURKEY | |
dc.relation.ispartof | TURKISH JOURNAL OF BIOLOGY | |
dc.subject | Platelets | |
dc.subject | apoptosis | |
dc.subject | TNF-alpha | |
dc.subject | iNOS | |
dc.subject | caspase-3 | |
dc.title | Tumor necrosis factor-alpha induced caspase-3 activation-related iNOS gene expression in ADP-activated platelets | |
dc.type | Article |