Effects of Duraseal (R) and Fibrin Glue on healing of normal and ischemic colon anastomosis

Abstract

Background and Aims: Anastomotic leaks represent a major complication of colorectal surgery. This study, involving a rat model of normal and ischemic colon anastomosis, aims to compare the effects of Duraseal (R) with those of Fibrin Glue (FG). Methods: Fifty adult male Wistar Albino rats were divided into six groups

normal colon anastomosis, ischemic colon anastomosis, FG on normal colon anastomosis, Duraseal (R) on normal colon anastomosis, FG on ischemic colon anastomosis, Duraseal (R) on ischemic colon anastomosis. After scarification, bursting pressure were measured and samples were collected for histopathological examination and hydroxyproline assays. Results: While the mean bursting pressure was statistically higher in groups treated with Duraseal (R) when compared to controls (p 0.05), no significant differences between Duraseal (R) and FG were detected (p 0.05). The mean hydroxyproline level was significantly lower in the Duraseal (R) groups than in the FG groups (p<0.05). However, significant differences between Duraseal (R) and control groups were found only in ischemic colon anastomosis (p<0.05). Histopathological examinations did not show any differences in wound healing. Conclusion: Considering the advantages associated with the use of Duraseal (R), we may assume that it may play role in gastrointestinal surgery with respect to prevention of anastomotic leaks. However, data is limited, and further studies are warranted to better define its place in surgery.