A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation

Simple item page
dc.contributor.authorMozioglu, Erkan
dc.contributor.authorAkgoz, Muslum
dc.contributor.authorTamerler, Candan
dc.contributor.authorKocagoz, Zuhtu Tanil
dc.date.accessioned2023-02-21T12:38:37Z
dc.date.available2023-02-21T12:38:37Z
dc.date.issued2014-01-01
dc.description.abstractA high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.
dc.description.issue1
dc.description.pages125-129
dc.description.volume38
dc.identifier.doi10.3906/biy-1308-7
dc.identifier.urihttps://hdl.handle.net/11443/2406
dc.identifier.urihttp://dx.doi.org/10.3906/biy-1308-7
dc.identifier.wosWOS:000329169300014
dc.publisherTUBITAK SCIENTIFIC \& TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGY
dc.subjectBacteria
dc.subjectgenomic DNA isolation
dc.subjectguanidinium isothiocyanate
dc.titleA simple guanidinium isothiocyanate method for bacterial genomic DNA isolation
dc.typeArticle

Files

Collections

WOS