Browsing by Author "Bolat, Ismail"

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    Potential Neurotoxic Effects of Glioblastoma-Derived Exosomes in Primary Cultures of Cerebellar Neurons via Oxidant Stress and Glutathione Depletion
    (MDPI, 2022-01-01) Genc, Sidika; Pennisi, Manuela; Yeni, Yesim; Yildirim, Serkan; Gattuso, Giuseppe; Altinoz, Meric A.; Taghizadehghalehjoughi, Ali; Bolat, Ismail; Tsatsakis, Aristidis; Hacimuftuoglu, Ahmet; Falzone, Luca
    High-grade gliomas are the most fatal brain tumors. Grade 4 gliomas are called glioblastoma multiforme (GBM), which are associated with the poorest survival and a 5-year survival rate of less than 4\%. Many patients with GBM developed concomitant cognitive dysfunctions and epilepsy. Although the cognitive decline is well defined in glioblastomas, the neurotoxic factors underlying this pathology are not well understood in GBM patients. In this study, we aimed to investigate whether GBM-derived exosomes play a role in neuronal toxicity. For this purpose, exosomes obtained from T98G and U373 GBM cells were applied to primary neuron culture at different concentrations. Subsequently, MTT, LDH, GSH, TAS, and TOS tests were performed. Both GBM-derived exosomes induced a dose-dependent and statistically significant increase of LDH release in cerebellar neurons. MTT assay revealed as both T98G and U373 GBM-derived exosomes induced dose-dependent neurotoxic effects in cerebellar neurons. To the best of our knowledge, this study is the first study demonstrating the toxic potential of GBM-derived exosomes to primary neurons, which may explain the peritumoral edema and cognitive decline in GBM patients.
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    Suboptimal use of non-vitamin K antagonist oral anticoagulants: Results from the RAMSES study
    (LIPPINCOTT WILLIAMS \& WILKINS, 2016-01-01) Basaran, Ozcan; Dogan, Volkan; Beton, Osman; Tekinalp, Mehmet; Aykan, Ahmet Cagri; Kalaycioglu, Ezgi; Bolat, Ismail; Tasar, Onur; Safak, Ozgen; Kalcik, Macit; Yaman, Mehmet; Inci, Sinan; Altintas, Bernas; Kalkan, Sedat; Kirma, Cevat; Biteker, Murat; Karadeniz, Fatma Ozpamuk; Tekkesin, Ahmet Ilker; Cakilli, Yasin; Turkkan, Ceyhan; Hamidi, Mehmet; Demir, Vahit; Gursoy, Mustafa Ozan; Ozturk, Mujgan Tek; Aksan, Gokhan; Seyis, Sabri; Balli, Mehmet; Alici, Mehmet Hayri; Bozyel, Serdar; Altun, Ibrahim; Calik, Feyza; Karaca, Oguz; Helvaci, Fusun; Akay, Kadriye; Canga, Yigit; Celebi, Savas; Altuntas, Emine; Ayturk, Mehmet; Gunes, Haci Murat; Bezgin, Tahir; Aksakal, Aytekin; Cakal, Beytullah; Colak, Ayse; Kaplan, Ozgur; Tatlisu, Adem; Gozubuyuk, Gokhan; Demirelli, Selami; Kaya, Adnan; Rencuzogullari, Ibrahim; Bayram, Zubeyde; Simsek, Zeki; Civan, Murat; Batgharel, Ulaankhu; Ata, Ali Ekber; Gol, Gokhan; Mert, Gurbet Ozge; Mert, Kadir Ugur; Degirmencioglu, Aleks; Candan, Ozkan; Celebi, Ozlem Ozcan; Dogan, Cem; Yavuz, Fethi; Ulucan, Seref; Arisoy, Arif; Sahin, Bingul Dilekci; Ermis, Emrah; Gokaslan, Serkan; Pektas, Idris; Tanindi, Asli; Tekin, Kamuran; Sancar, Kadriye Memic; Cekic, Edip Guvenc; Basaran, Nesrin Filiz
    This study aimed to investigate the potential misuse of novel oral anticoagulants (NOACs) and the physicians' adherence to current European guideline recommendations in real-world using a large dataset from Real-life Multicenter Survey Evaluating Stroke Prevention Strategies in Turkey (RAMSES Study).RAMSES study is a prospective, multicenter, nationwide registry (ClinicalTrials.gov identifier NCT02344901). In this subgroup analysis of RAMSES study, patients who were on NOACs were classified as appropriately treated (AT), undertreated (UT), and overtreated (OT) according to the European Society of Cardiology (ESC) guidelines. The independent predictors of UT and OT were determined by multivariate logistic regression.Of the 2086 eligible patients, 1247 (59.8\%) received adequate treatment. However, off-label use was detected in 839 (40.2\%) patients