Browsing by Author "Ciraci, Mehmet Zahid"

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    Evaluation of optimal urine screening and confirmation cut-off values for opiates, at a national reference laboratory
    (WALTER DE GRUYTER GMBH, 2021-01-01) Karakukcu, Cigdem; Ciraci, Mehmet Zahid; Kocer, Derya; Faydali, Mine Yuce; Serdar, Muhittin Abdulkadir
    Objectives: To obtain optimal immunoassay screening and LC-MS/MS confirmation cut-offs for opiate group tests to reduce false positive (FP) and false negative (FN) rates. Methods: A total of 126 urine samples, -50 opiate screening negative, 76 positive according to the threshold of 300 ng/mL by CEDIA method - were confirmed by a full-validated in-house LC-MS/MS method. Sensitivity, specificity, FP, and FN rates were determined at cut-off concentrations of both 300 and 2,000 ng/mL formorphine and codeine, and 10 ng/mLfor heroinmetabolite 6-mono-acetyl-morphine (6-MAM). Results: All CEDIA opiate negative urine samples were negative for morphine, codeine and 6-MAM. Although sensitivity was 100\% for each cut-off