Browsing by Author "Durer, Zeynep A. Oztug"

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    D-loop Dynamics and Near-Atomic-Resolution Cryo-EM Structure of Phalloidin-Bound F-Actin
    (CELL PRESS, 2020-01-01) Das, Sanchaita; Ge, Peng; Durer, Zeynep A. Oztug; Grintsevich, Elena E.; Zhou, Z. Hong; Reisler, Emil
    Detailed molecular information on G-actin assembly into filaments (F-actin), and their structure, dynamics, and interactions, is essential for understanding their cellular functions. Previous studies indicate that a flexible DNase I binding loop (D-loop, residues 40-50) plays a major role in actin's conformational dynamics. Phalloidin, a ``gold standard{''} for actin filament staining, stabilizes them and affects the D-loop. Using disulfide crosslinking in yeast actin D-loop mutant Q41C/V45C, light-scattering measurements, and cryoelectron microscopy reconstructions, we probed the constraints of D-loop dynamics and its contribution to F-actin formation/stability. Our data support a model of residues 41-45 distances that facilitate G- to F-actin transition. We report also a 3.3-angstrom resolution structure of phalloidin-bound F-actin in the ADP-Pi-like (ADP-BeFx) state. This shows the phalloidin-binding site on F-actin and how the relative movement between its two protofilaments is restricted by it. Together, our results provide molecular details of F-actin structure and D-loop dynamics.
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    Drosophila and human FHOD family formin proteins nucleate actin filaments
    (AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, 2018-01-01) Patel, Aanand A.; Durer, Zeynep A. Oztug; van Loon, Aaron P.; Bremer, Kathryn V.; Quinlan, Margot E.
    Formins are a conserved group of proteins that nucleate and processively elongate actin filaments. Among them, the formin homology domain-containing protein (FHOD) family of formins contributes to contractility of striated muscle and cell motility in several contexts. However, the mechanisms by which they carry out these functions remain poorly understood. Mammalian FHOD proteins were reported not to accelerate actin assembly in vitro
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    The Drosophila Formin FHOD Nucleates Actin Filaments
    (2017-01-01) Patel, Aanand A.; Durer, Zeynep A. Oztug; van Loon, Aaron P.; Bremer, Kathryn V.; Quinlan, Margot E.