Browsing by Author "Kocagoz, S."

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    Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species of mycobacteria other than tuberculosis grown in Lowenstein-Jensen and TK-SLC medium
    (ELSEVIER, 2010-01-01) Akyar, I.; Kocagoz, T.; Sinik, G.; Oktem, S.; Aytekin, N.; Kocagoz, S.
    Background: Mycobacterial antigen MPB64 is a secretory protein specific for Mycobacterium tuberculosis complex. A lateral flow immunochromatographic assay (ICA) is a method used for the rapid differentiation of M. tuberculosis complex. Aim: We aimed to evaluate the performance of ICA in rapid differentiation of M. tuberculosis complex from 97 Mycobacterium species other than tuberculosis (MOTT), which are grown in Lowenstein-Jensen and TK-selective (SLC) medium. Materials and Methods: The study was performed in our laboratory between January 2009 and January 2010. A total of 394 isolates consisting of reference strains of 34 M. tuberculosis from World Health Organization (WHO) collection, 97 different MOTT bacilli, 7 Mycobacterium bovis BCG substrains and total 256 clinical Mycobacterium isolates were tested by ICA, which is based on anti-MPB64 monoclonal antibodies. All the strains were inoculated onto a TK-SLC (selective) medium and Lowenstein-Jensen medium. TK-SLC is a new rapid mycobacterial culture medium that indicates mycobacterial growth by colour change. Results: The growth of mycobacterial strains was observed in 10-12 days on TK-SLC medium. ICA test was performed in 15 minutes. All strains belonging to M. tuberculosis complex group were found positive and all MOTT species were found negative on ICA slides. The results were confirmed with nucleic acid amplification by polymerase chain reaction (PCR) using primers specific for M. tuberculosis complex. Conclusion: With the additive effect of growth on TK-SLC medium in 10-12 days, the mycobacterial antigen MPB64 is a very useful and specific tool in rapid differentiation of M. tuberculosis and MOTT grown in culture.
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    The course of spinal tuberculosis (Pott disease): results of the multinational, multicentre Backbone-2 study
    (ELSEVIER SCI LTD, 2015-01-01) Batirel, A.; Erdem, H.; Sengoz, G.; Pehlivanoglu, F.; Ramosaco, E.; Gulsun, S.; Tekin, R.; Mete, B.; Balkan, I. I.; Sevgi, D. Y.; Giannitsioti, E.; Fragou, A.; Kaya, S.; Cetin, B.; Oktenoglu, T.; Celik, A. D.; Karaca, B.; Horasan, E. S.; Ulug, M.; Senbayrak, S.; Kaya, S.; Arslanalp, E.; Hasbun, R.; Ates-Guler, S.; Willke, A.; Senol, S.; Inan, D.; Guclu, E.; Ertem, G. T.; Koc, M. M.; Tasbakan, M.; Ocal, G.; Kocagoz, S.; Kusoglu, H.; Guven, T.; Baran, A. I.; Dede, B.; Karadag, F. Y.; Yilmaz, H.; Aslan, G.; Al-Gallad, D. A.; Cesur, S.; El-Sokkary, R.; Sirmatel, F.; Savasci, U.; Karaahmetoglu, G.; Vahaboglu, H.
    We aimed to describe clinical, laboratory, diagnostic and therapeutic features of spinal tuberculosis (ST), also known as Pott disease. A total of 314 patients with ST from 35 centres in Turkey, Egypt, Albania and Greece were included. Median duration from initial symptoms to the time of diagnosis was 78 days. The most common complications presented before diagnosis were abscesses (69\%), neurologic deficits (40\%), spinal instability (21\%) and spinal deformity (16\%). Lumbar (56\%), thoracic (49\%) and thoracolumbar (13\%) vertebrae were the most commonly involved sites of infection. Although 51\% of the patients had multiple levels of vertebral involvement, 8\% had noncontiguous involvement of multiple vertebral bodies. The causative agent was identified in 41\% of cases. Histopathologic examination was performed in 200 patients (64\%), and 74\% were consistent with tuberculosis. Medical treatment alone was implemented in 103 patients (33\%), while 211 patients (67\%) underwent diagnostic and/or therapeutic surgical intervention. Ten percent of the patients required more than one surgical intervention. Mortality occurred in 7 patients (2\%), and 77 (25\%) developed sequelae. The distribution of the posttreatment sequelae were as follows: 11\% kyphosis, 6\% Gibbus deformity, 5\% scoliosis, 5\% paraparesis, 5\% paraplegia and 4\% loss of sensation. Older age, presence of neurologic deficit and spinal deformity were predictors of unfavourable outcome. ST results in significant morbidity as a result of its insidious course and delayed diagnosis because of diagnostic and therapeutic challenges. ST should be considered in the differential diagnosis of patients with vertebral osteomyelitis, especially in tuberculosis-endemic regions. Early establishment of definitive aetiologic diagnosis and appropriate treatment are of paramount importance to prevent development of sequelae. Clinical Microbiology and Infection (C) 2015 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.