Browsing by Author "Sipahi, Hande"

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    Antimicrobial, Cytotoxic, Antiviral Effects, and Spectroscopic Characterization of Metabolites Produced by Fusarium oxysporum YP9B
    (2021-01-01) Kilic, Gozde; Tosun, Gonca; Bozdeveci, Arif; Erik, Ishak; Ozturk, Elif; Reis, Rengin; Sipahi, Hande; Cora, Merve; Karaoglu, Sengul Alpay; Yayli, Nurettin
    The goal of the work is to determine the bioactive pharmaceutical metabolites produced by the Fusarium oxysporum YP9B isolate. Ten new natural compounds were isolated from the ethyl acetate extract of the F. oxysporum YP9B strain. Their structures were elucidated by spectroscopic methods using 1D and 2D NMR, UV, FT-IR, and mass spectra (LC-QTOF MS and GC-FID/MS). Identified compounds were named as
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    Assessment of dermal biocompatibility and antimicrobial activity of silver-made nipple cap
    (MARMARA UNIV, 2021-01-01) Charehsaz, Mohammad; Reis, Rengin; Sumer, Engin; Orak, Duygu; Deniz, Inci; Sipahi, Hande; Aydin, Ahmet
    Breastfeeding is considered a gold standard for infant development. However, several complications such as nipple fissure, infection and ulceration might be accomplished during nursing, which may lead to early cessation of breastfeeding. For this purpose, silver-made nipple cap SilverNurse (R) (SN) has been designed to control symptoms of nipple fissure in lactating women by aiming to eliminate antimicrobial resistance compared to the alternative treatment methods. Therefore, aim of the present study is to assess antimicrobial efficacy and dermal safety of silver-made SN in context of biocompatibility with cytotoxicity assay, in vitro EpiDetin skin irritation and in vivo skin irritation tests. According to the represented results, SN exhibited potent antimicrobial activity on Staphylococcus aureus, Escherichia soli, Enterococcus hirae, and Pseudonionas aeruginosa bacteria as well as Candida albicans fungi. Cytotoxicity by MTT assay showed that prepared extract of SN has a lack of cytotoxicity in tested concentrations. In addition, in vitro EpiDerm skin irritation model and in vivo skin test demonstrated that SN can be classified as biocompatible and non-irritant for dermal use without any sign of erythema and edema. Therefore, in the present study, it was evaluated that SN can be applied safely in daily comfort fields for lactating women without dermal complications related to infection and skin irritation.
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    Secondary metabolites from Gentiana cruciata L. and their anti-inflammatory and analgesic activities
    (TAYLOR \& FRANCIS LTD, 2022-01-01) Konya, Rima; Reis, Rengin; Sipahi, Hande; Barta, Anita; Hohmann, Judit; Kirmizibekmez, Hasan
    A previously unreported secoiridoid glycoside, cruciatoside (1) was isolated from the aerial parts of Gentiana cruciata L. along with ten known compounds eustomoside (2), eustomorusside (3), gentiopicroside (4), 6'-O-beta-D-glucopyranosyl gentiopicroside (5), loganic acid (6), isoorientin (7), isovitexin (8), isovitexin 2''-(E)-ferulate (9), mangiferin (10), and 2-methyl-inositol (11). The chemical structures of the isolates were elucidated based on extensive 1 D and 2 D NMR experiments as well as HRMS analysis. All isolates were evaluated for their in vitro anti-inflammatory and analgesic activities. Compounds 9, 4, and 7 (200 mu M) showed moderate anti-inflammatory activity by inhibiting nitrite production from LPS-induced RAW 264.7 macrophage cells, with the inhibition rates of 39.5\%, 25.8\% and 22.9\% respectively without exhibiting substantial cytotoxicity. Besides, 1, 2, 4, and 7 exerted the highest decrease in IL-6 levels. Moreover, compound 4 showed in vitro analgesic activity by decreasing the PGE(2) level comparable to the reference drugs.