Browsing by Author "Sirvanci, Serap"

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    Investigation of Neurogenesis in Kindled Wistar and Genetic Absence Epilepsy Rats
    (MARMARA UNIV, INST HEALTH SCIENCES, 2022-01-01) Kandemir, Cansu; Yavuz, Melis; Karakaya, Fatma Bedia; Kaya, Ozlem Tugce Cilingir; Onat, Filiz; Sirvanci, Serap
    Objective: The most common type of epilepsy affecting about 50 million people worldwide is temporal lobe epilepsy (TLE). Chemical and electrical kindling methods in animals can be used to form TLE model. In the present study, it was aimed to investigate neurogenesis in the hippocampus of adult kindled Wistar rats and genetic absence epilepsy rats from Strasbourg (GAERS) rats by immunofluorescence methods.Methods: Adult Wistar and GAERS albino rats weighing 250-300 gr were injected pentylenetetrazole (PTZ) (35 mg/kg, s.c.) every other day to produce chemical kindling. Animals having 5 times grade 5 seizures were considered to be kindled. Intracardiac perfusion was performed under deep anesthesia on the 7th and 14th days after the last grade 5 seizure. Immunofluorescence methods were used to demonstrate newly formed neurons, astroglial cells, and mature neurons, by using anti-doublecortin (DCX), anti-glial fibrillary acidic protein (GFAP), and antineuronal nuclear antigen (NeuN) primary antibodies, respectively. Sections were then examined under a fluorescence microscope.Results: DCX (+) cells were found to be increased in GAERS control groups compared to the Wistar control groups
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    Loop nerve graft prefabrication for peripheral nerve defect reconstruction
    (TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2022-01-01) Oksuz, Sinan; Eren, Fikret; Cesur, Ceyhun; Elmas, Merve Acikel; Sirvanci, Serap
    BACKGROUND: Delayed autologous nerve graft reconstruction is inevitable in devastating injuries. Delayed or prolonged repair time has deleterious effects on nerve grafts. We aimed improving and accelerating nerve graft reconstruction process in a rat long nerve defect model with loop nerve graft prefabrication particularly to utilize for injuries with tissue loss. METHODS Twenty-four Sprague-Dawley rats were allocated into three groups. 1.5 cm long peroneal nerve segment was excised, reversed in orientation, and used as autologous nerve graft. In conventional interpositional nerve graft group (Group 1), nerve defects were repaired in single-stage. In loop nerve graft prefabrication group (Group 2), grafts were sutured end-to-end (ETE) to the proximal peroneal nerve stumps. Distal ends of the grafts were sutured end-to-side to the peroneal nerve stumps 5 mm proximal to the ETE repair sites in first stage. In second stage, distal ends of the prefabricated grafts were transposed and sutured to distal nerve stumps. In staged conventional interpositional nerve graft group (Group 3), grafts were sutured ETE to proximal peroneal nerve stumps in first stage. Distal ends of the grafts and nerve stumps were tacked to the surrounding muscles until the final repair in second stage. Followup period was 4 weeks for each stage in Groups 2 and 3, and 8 weeks for Group 1. Peroneal function index (PFI), electrophysiology, and histological assessments were conducted after 8 weeks. P<0.05 was considered significant for statistical analysis. RESULTS: PFI results of Group 1 (-22.75 +/- 5.76) and 2 (-22.08 +/- 6) did not show statistical difference (p>0.05). Group 3 (-33.64 +/- 6.4) had a statistical difference compared to other groups (p<0.05). Electrophysiology results of Group 1 (16.19 +/- 2.15 mV/1.16 +/- 0.21 ms) and 2 (15.95 +/- 2.82 mV/1.17 +/- 0.16 ms) did not present statistical difference (p>0.05), whereas both groups had a statistical difference compared to Group 3 (10.44 +/- 1.96 mV/ 1.51 +/- 0.15 ms) (p<0.05). Axon counts of Group 1 (2227 +/- 260.4) and 3 (2194 +/- 201.1) did not have statistical difference (p>0.05), whereas both groups had significantly poor axon counts compared to Group 2 (2531 +/- 91.18) (p<0.05). CONCLUSION. Loop nerve graft prefabrication improved axonal regeneration without delay. Loop prefabrication can accelerate prolonged regeneration time for the injuries indicating a delayed nerve reconstruction. Higher axon counts derived with loop nerve prefabrication may even foster its investigation in immediate long nerve defect reconstructions in further studies.