Proteins associated with neutrophil degranulation are upregulated in nasopharyngeal swabs from SARS-CoV-2 patients

dc.contributor.authorAkgun, Emel
dc.contributor.authorTuzuner, Mete Bora
dc.contributor.authorSahin, Betul
dc.contributor.authorKilercik, Meltem
dc.contributor.authorKulah, Canan
dc.contributor.authorCakiroglu, Hacer Nur
dc.contributor.authorSerteser, Mustafa
dc.contributor.authorUnsal, Ibrahim
dc.contributor.authorBaykal, Ahmet Tarik
dc.date.accessioned2023-02-21T12:34:03Z
dc.date.available2023-02-21T12:34:03Z
dc.date.issued2020-01-01
dc.description.abstractCOVID-19 or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appeared throughout the World and currently affected more than 9 million people and caused the death of around 470,000 patients. The novel strain of the coronavirus disease is transmittable at a devastating rate with a high rate of severe hospitalization even more so for the elderly population. Naso-oro-pharyngeal swab samples as the first step towards detecting suspected infection of SARS-CoV-2 provides a non-invasive method for PCR testing at a high confidence rate. Furthermore, proteomics analysis of PCR positive and negative naso-oropharyngeal samples provides information on the molecular level which highlights disease pathology. Samples from 15 PCR positive cases and 15 PCR negative cases were analyzed with nanoLC-MS/MS to identify the differentially expressed proteins. Proteomic analyses identified 207 proteins across the sample set and 17 of them were statistically significant. Protein-protein interaction analyses emphasized pathways like Neutrophil degranulation, Innate Immune System, Antimicrobial Peptides. Neutrophil Elastase (ELANE), Azurocidin (AZU1), Myeloperoxidase (MPO), Myeloblastin (PRTN3), Cathepsin G (CTSG) and Transcobalamine-1 (TCN1) were found to be significantly altered in naso-oropharyngeal samples of SARS-CoV-2 patients. The identified proteins are linked to alteration in the innate immune system specifically via neutrophil degranulation and NETosis.
dc.description.issue10
dc.description.issueOCT 20
dc.description.volume15
dc.identifier.doi10.1371/journal.pone.0240012
dc.identifier.urihttps://hdl.handle.net/11443/1651
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0240012
dc.identifier.wosWOS:000585940600016
dc.publisherPUBLIC LIBRARY SCIENCE
dc.relation.ispartofPLOS ONE
dc.titleProteins associated with neutrophil degranulation are upregulated in nasopharyngeal swabs from SARS-CoV-2 patients
dc.typeArticle

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