The importance of histological patterns on PD-L1 staining heterogeneity: Should we use pattern-based approach for selecting tumor samples for PD-L1 testing in lung

dc.contributor.authorBulutay, Pinar
dc.contributor.authorFirat, Pinar
dc.contributor.authorZeren, Handan
dc.contributor.authorErus, Suat
dc.contributor.authorTanju, Serhan
dc.contributor.authorDilege, Sukru
dc.date.accessioned2023-02-21T12:33:18Z
dc.date.available2023-02-21T12:33:18Z
dc.date.issued2021-01-01
dc.description.abstractBackground/aim: Programmed death ligand-1 (PD-L1) is a predictive marker for immunotherapeutic agents. However, heterogeneous staining of PD-L1 can cause false-negative results. The aim of this study is to evaluate the importance of histological patterns on PD-L1 staining heterogeneity in lung adenocarcinomas (LAC). Materials and methods: PD-L1 immunohistochemistry (IHC) stain was performed to two different tissue cores of 128 LAC cases, and cut-off values are given for grouping the cases according to the percentage of staining (1\%-10\%, 11\%-49\%, 50\%-100\%). Staining rates between cores were compared and analyzed by their histological patterns. Also, the relation of the PD-L1 expression with the clinicopathological characteristics of the cases was analyzed. Results: Overall, PD-L1 expression was observed in 53 of 128 cases (41.4\%, 1\% cut-off), 23.5\% of them were positive at 10\% cut-off and 14.1\% at 50\% cut-off. PD-L1 expression was significantly related to the high grade micropapillary and solid patterns of adenocarcinomas (p:0.01). Staining cut-offs were mostly similar between cores (43/50, 86\%) (k:0.843). However, 14\% of them were positive only in one core (7 of 50). This false negativity was mostly related to the histological patterns. Conclusion: Our data reveal the heterogeneous staining of PD-L1 expression, also micropapillary and solid patterns show higher rates of PDL expression. Therewithal, these findings also highlight the importance of taking into consideration of histological patterns, when choosing a paraffin block for the PDL1.
dc.description.issue1
dc.description.pages204-213
dc.description.volume51
dc.identifier.doi10.3906/sag-2004-61
dc.identifier.urihttps://hdl.handle.net/11443/1452
dc.identifier.urihttp://dx.doi.org/10.3906/sag-2004-61
dc.identifier.wosWOS:000623196400026
dc.publisherTUBITAK SCIENTIFIC \& TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF MEDICAL SCIENCES
dc.subjectPD-L1
dc.subjectlung cancer
dc.subjectimmunotherapy
dc.subjectlung adenocarcinoma
dc.titleThe importance of histological patterns on PD-L1 staining heterogeneity: Should we use pattern-based approach for selecting tumor samples for PD-L1 testing in lung
dc.typeArticle

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