Brain region-specific amyloid plaque-associated myelin lipid loss, APOE deposition and disruption of the myelin sheath in familial Alzheimer's disease mice

dc.contributor.authorKaya, Ibrahim
dc.contributor.authorJennische, Eva
dc.contributor.authorLange, Stefan
dc.contributor.authorBaykal, Ahmet Tarik
dc.contributor.authorMalmberg, Per
dc.contributor.authorFletcher, John S.
dc.date.accessioned2023-02-21T12:34:26Z
dc.date.available2023-02-21T12:34:26Z
dc.date.issued2020-01-01
dc.description.abstractThere is emerging evidence that amyloid beta (A beta) aggregates forming neuritic plaques lead to impairment of the lipid-rich myelin sheath and glia. In this study, we examined focal myelin lipid alterations and the disruption of the myelin sheath associated with amyloid plaques in a widely used familial Alzheimer's disease (AD) mouse model
dc.description.abstract5xFAD. This AD mouse model has A beta(42) peptide-rich plaque deposition in the brain parenchyma. Matrix-assisted laser desorption/ionization imaging mass spectrometry of coronal brain tissue sections revealed focal A beta plaque-associated depletion of multiple myelin-associated lipid species including sulfatides, galactosylceramides, and specific plasmalogen phopshatidylethanolamines in the hippocampus, cortex, and on the edges of corpus callosum. Certain phosphatidylcholines abundant in myelin were also depleted in amyloid plaques on the edges of corpus callosum. Further, lysophosphatidylethanolamines and lysophosphatidylcholines, implicated in neuroinflammation, were found to accumulate in amyloid plaques. Double staining of the consecutive sections with fluoromyelin and amyloid-specific antibody revealed amyloid plaque-associated myelin sheath disruption on the edges of the corpus callosum which is specifically correlated with plaque-associated myelin lipid loss only in this region. Further, apolipoprotein E, which is implicated in depletion of sulfatides in AD brain, is deposited in all the A beta plaques which suggest apolipoprotein E might mediate sulfatide depletion as a consequence of an immune response to A beta deposition. This high-spatial resolution matrix-assisted laser desorption/ionization imaging mass spectrometry study in combination with (immuno) fluorescence staining of 5xFAD mouse brain provides new understanding of morphological, molecular and immune signatures of A beta plaque pathology-associated myelin lipid loss and myelin degeneration in a brain region-specific manner.
dc.description.issue1
dc.description.issueJUL
dc.description.pages84-98
dc.description.volume154
dc.identifier.doi10.1111/jnc.14999
dc.identifier.urihttps://hdl.handle.net/11443/1741
dc.identifier.urihttp://dx.doi.org/10.1111/jnc.14999
dc.identifier.wosWOS:000521414900001
dc.publisherWILEY
dc.relation.ispartofJOURNAL OF NEUROCHEMISTRY
dc.subjectAlzheimer's disease
dc.subjectamyloid plaques
dc.subjectapolipoprotein E (APOE)
dc.subjectMALDI Imaging Mass Spectrometry
dc.subjectmyelin lipids
dc.subjectsulfatides
dc.titleBrain region-specific amyloid plaque-associated myelin lipid loss, APOE deposition and disruption of the myelin sheath in familial Alzheimer's disease mice
dc.typeArticle

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