Biological variations of ADAMTS13 and von Willebrand factor in human adults

dc.contributor.authorKilercik, Meltem
dc.contributor.authorCoskun, Abdurrahman
dc.contributor.authorSerteser, Mustafa
dc.contributor.authorInan, Deniz
dc.contributor.authorUnsal, Ibrahim
dc.date.accessioned2023-02-21T12:33:38Z
dc.date.available2023-02-21T12:33:38Z
dc.date.issued2014-01-01
dc.description.abstractBackground: The ultra-large von Willebrand factor (vWF) multimers are very active and must be degraded by ADAMTS13 for optimal activity. A severe functional deficiency of ADAMTS13 has been associated with thrombotic thrombocytopenic purpura. The correct interpretation of patient vWF and ADAMTS13 plasma levels requires an understanding of the biological variation associated with these analytes. In the present paper, we aimed to determine the biological variation of ADAMTS13 and vWF in human adults. Materials and methods: Blood samples were collected weekly from 19 healthy subjects for 5 consecutive weeks. vWF activity and antigenicity were determined using aggregometric and immunoturbidimetric methods. ADAMTS13 antigenicity and activity were determined by ELISA. Results: The within-subject biological variations for vWF activity and antigenicity were 8.06\% and 14.37\%, respectively, while the between-subject biological variations were 18.5\% and 22.59\%, respectively. The index of individuality for vWF activity was 0.44, while vWF antigenicity was 0.64. Similarly, ADAMTS13 activity and antigenicity within-subject biological variations were 12.73\% and 9.75\%, respectively, while between-subject biological variations were 9.63\% and 6.28\%, respectively. The ADAMTS13 indexes of individuality were 1.32 and 1.55, respectively. Conclusion: We report high biological variation and individuality in vWF antigenicity and activity levels. However, ADAMTS13 antigenicity and activity displayed high biological variation, but low individuality. Thus, population-based reference intervals may be useful for monitoring ADAMTS13 antigenicity and activity, but not for vWF, which displays high individuality. These findings should be considered when determining the reference interval and other clinical variables associated with ADAMTS13 and vWF levels.
dc.description.issue1
dc.description.pages138-145
dc.description.volume24
dc.identifier.urihttps://hdl.handle.net/11443/1545
dc.identifier.wosWOS:000331269800016
dc.publisherCROATIAN SOC MEDICAL BIOCHEMISTS
dc.relation.ispartofBIOCHEMIA MEDICA
dc.subjectADAMTS13
dc.subjectvariation analysis
dc.subjectindividuality
dc.subjectthrombotic thrombocytopenic purpura
dc.subjectvon Willebrand factor
dc.titleBiological variations of ADAMTS13 and von Willebrand factor in human adults
dc.typeArticle

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