Hairpin structures with conserved sequence motifs determine the 3 ` ends of non-polyadenylated invertebrate iridovirus transcripts
Hairpin structures with conserved sequence motifs determine the 3 ` ends of non-polyadenylated invertebrate iridovirus transcripts
Tarih
2017-01-01
Yazarlar
Ince, Ikbal Agah
Pijlman, Gorben P.
Vlak, Just M.
van Oers, Monique M.
Süreli Yayın başlığı
Süreli Yayın ISSN
Cilt Başlığı
Yayınevi
ACADEMIC PRESS INC ELSEVIER SCIENCE
Dergi Adı
VIROLOGY
Özet
Previously, we observed that the transcripts of Invertebrate iridescent virus 6 (IIV6) are not polyadenylated, in line with the absence of canonical poly(A) motifs (AATAAA) downstream of the open reading frames (ORFs) in the genome. Here, we determined the 3' ends of the transcripts of fifty-four IIV6 virion protein genes in infected Drosophila Schneider 2 (52) cells. By using ligation-based amplification of cDNA ends (LACE) it was shown that the IIV6 mRNAs often ended with a CAUUA motif. In silico analysis showed that the 3'-untranslated regions of IIV6 genes have the ability to form hairpin structures (22-56 nt in length) and that for about half of all IIV6 genes these 3' sequences contained complementary TAATG and CATTA motifs. We also show that a hairpin in the 3' flanking region with conserved sequence motifs is a conserved feature in invertebrate-infecting iridoviruses (genus Iridovirus and Chloriridovirus).
Açıklama
Anahtar kelimeler
Invertebrate iridescent virus, Chilo iridescent virus, Iridovirus, RNA processing, mRNA termination, Non-polyadenylated transcripts, LACE, Hairpin structures, Inverted repeats