Investigation of ACE Genome Insertion/Deletion Correlation with Immunohistochemical Profile in Pituitary Adenomas
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Date
Journal Title
Journal ISSN
Volume Title
dc.identifier.issn
dc.identifier.doi
10.5137/1019-5149.JTN.7423-12.0
dc.identifier.other
dc.description.volume
23
dc.description.issue
4
JUL
JUL
Publisher
TURKISH NEUROSURGICAL SOC
DOI
10.5137/1019-5149.JTN.7423-12.0
Abstract
AIM: The deletion polymorphism of the angiotensin-converting enzyme (ACE) genome causes neoplastic development in several organs by increasing the angiotensin 2 (A2) formation. In this study, we aimed to identify the ACE genome insertion/deletion polymorphism in pituitary adenomas and to compare it with the control group. MATERIAL and METHODS: Patients operated for pituitary adenomas were included in the study. Genomic DNA was extracted from tumoral tissues and peripheral blood samples of the patients by using the Miller method. Primary sequence was selected via targeting the polymorphic region of intron 16 of ACE genome 17q23. DNA samples were multiplied by PCR using HACE3s and HACE3as primers. RESULTS: Twenty-one operated cases were studied. In the study group
44\% of the patients were identified as DID, 33\% of them as I/D and 23\% of them as I/I. In 60\%, D allele was identified. According to immunohistochemical investigation, we found that 100\% of the patients with Cushing adenoma were D/D alleles. CONCLUSION: Presence of high rate of ACE genome deletion in patients with pituitary adenoma and grade 3-4 patients suggest that ACE genome polymorphism can be a risk factor for the development of pituitary adenomas.
44\% of the patients were identified as DID, 33\% of them as I/D and 23\% of them as I/I. In 60\%, D allele was identified. According to immunohistochemical investigation, we found that 100\% of the patients with Cushing adenoma were D/D alleles. CONCLUSION: Presence of high rate of ACE genome deletion in patients with pituitary adenoma and grade 3-4 patients suggest that ACE genome polymorphism can be a risk factor for the development of pituitary adenomas.