Muscarinic agonist, antagonists and signaling pathway inhibitors change c-Fos and cyclin D-1 expression in K562 cells
dc.contributor.author | Cabadak, Hulya | |
dc.contributor.author | Aydin, Banu | |
dc.contributor.author | Kan, Beki | |
dc.date.accessioned | 2023-02-21T12:32:46Z | |
dc.date.available | 2023-02-21T12:32:46Z | |
dc.date.issued | 2013-01-01 | |
dc.description.abstract | Objectives: Muscarinic acetylcholine receptors (mAChR) belong to a family of G protein coupled receptors (GPCRs). These mAChRs regulate several important physiological functions by activating a wide variety of cellular signaling pathways. We have previously shown that muscarinic acetylcholine (M-2, M-3 and M-4) receptors are expressed in K562 cells. In this study, we investigated the effect of muscarinic agonist, antagonists and different signaling pathway inhibitors on c-Fos and cyclin D-1 transcripts, using reverse transcriptase polymerase chain reaction (RT-PCR) that allows changes of very rare transcripts to be monitored. Material and Methods: Total RNA was prepared from K562 cells challenged with muscarinic agonist, antagonists and inhibitors. c-Fos and cyclin D-1 expression were determined by RT-PCR. Results: We showed that treatment with muscarinic agonist, antagonists and inhibitors leads to changes in c-Fos and cyclin D-1 expression in K562 cells. Conclusions: Our results suggest that muscarinic receptors regulate expression of c-Fos and cyclin D-1 genes in K562 cells via different signaling pathways. | |
dc.description.issue | 2 | |
dc.description.pages | 72-76 | |
dc.description.volume | 26 | |
dc.identifier.doi | 10.5472/MMJ.2013.02807.1 | |
dc.identifier.uri | https://hdl.handle.net/11443/1260 | |
dc.identifier.uri | http://dx.doi.org/10.5472/MMJ.2013.02807.1 | |
dc.identifier.wos | WOS:000219997500005 | |
dc.publisher | MARMARA UNIV, FAC MEDICINE | |
dc.relation.ispartof | MARMARA MEDICAL JOURNAL | |
dc.subject | Atropine | |
dc.subject | Carbachol (CCh) | |
dc.subject | c-Fos | |
dc.subject | Cyclin D-1 | |
dc.title | Muscarinic agonist, antagonists and signaling pathway inhibitors change c-Fos and cyclin D-1 expression in K562 cells | |
dc.type | Article |