Differential gene expression analysis of human cumulus cells

dc.contributor.authorDemiray, Sirin Bakti
dc.contributor.authorGoker, Ege Nazan Tavmergen
dc.contributor.authorTavmergen, Erol
dc.contributor.authorYilmaz, Ozlem
dc.contributor.authorCalimlioglu, Nilufer
dc.contributor.authorSoykam, Huseyin Okan
dc.contributor.authorOktem, Gulperi
dc.contributor.authorSezerman, Ugur
dc.date.accessioned2023-02-21T12:35:47Z
dc.date.available2023-02-21T12:35:47Z
dc.date.issued2019-01-01
dc.description.abstractObjective: This study was performed to explore the possibility that each oocyte and its surrounding cumulus cells might have different genetic expression patterns that could affect human reproduction. Methods: Differential gene expression analysis was performed for 10 clusters of cumulus cells obtained from 10 cumulus-oocyte complexes from 10 patients. Same procedures related to oocyte maturation, microinjection, and microarray analyses were performed for each group of cumulus cells. Two differential gene expression analyses were performed: one for the outcome of clinical pregnancy and one for the outcome of live birth. Results: Significant genes resulting from these analyses were selected and the top 20 affected pathways in each group were analyzed. Circadian entrainment is determined to be the most affected pathway for clinical pregnancy, and proteoglycans in cancer pathway is the most affected pathway for live birth. Circadian entrainment is also amongst the 12 pathways that are found to be in top 20 affected pathways for both outcomes, and has both lowest p-value and highest number of times found count. Conclusion: Although further confirmatory studies are necessary, findings of this study suggest that these pathways, especially circadian entrainment in cumulus cells, may be essential for embryo development and pregnancy.
dc.description.issue2
dc.description.issueJUN
dc.description.pages76-86
dc.description.volume46
dc.identifier.doi10.5653/cerm.2019.46.2.76
dc.identifier.urihttps://hdl.handle.net/11443/1996
dc.identifier.urihttp://dx.doi.org/10.5653/cerm.2019.46.2.76
dc.identifier.wosWOS:000488246200005
dc.publisherKOREAN SOC REPRODUCTIVE MEDICINE
dc.relation.ispartofCLINICAL AND EXPERIMENTAL REPRODUCTIVE MEDICINE-CERM
dc.subjectAssisted reproductive techniques
dc.subjectCircadian clocks
dc.subjectCumulus cells
dc.subjectGranulosa cells
dc.subjectInfertility
dc.subjectLive birth
dc.subjectMicroarray analysis
dc.subjectOvarian follicle
dc.subjectPregnancy
dc.subjectProteoglycans
dc.titleDifferential gene expression analysis of human cumulus cells
dc.typeArticle

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