Analysis of Correlation between the Seven Important Helicobacter pylori (H. pylori) Virulence Factors and Drug Resistance in Patients with Gastritis

Abstract

The aim of this study is to evaluate the association between seven importantH.pylorivirulence factors and antibiotic resistance in patients with gastritis.H.pyloristrains isolated from 33 patients with gastritis were examined. Antimicrobial susceptibilities were tested by GenoType (R) HelicoDR (Hain Life Science, Germany) test kit and RT-PCR. The virulence-factors were determined using conventional PCR. 39\% of patients were resistant for clarithromycin and 27\% of patients were resistant for fluoroquinolone. 15\% of patients were resistant to both clarithromycin and fluoroquinolone. TheH.pylori vacA m1/s2genotype was the most frequent allelic combination. Patients were possessed thevacA s1,m1(6.1\%)s1,m2(6.1\%)s2,m1(15.1\%)ands2,m2(3.0\%) genotypes. 94\% of patients with gastritis were positive forH.pylori napAgene. Also, there were nodupAgene-positive gastritis patients. There was no significant correlation between thevacA,cagA,oipA,hpaA,babA,napA,dupA,ureA,ureBvirulence genes, clarithromycin, and fluoroquinolone resistance. Herein, we report that the relationship between theH.pylori napAgene and gastritis. Although we found a correlation betweenH.pylorivirulence factor and clinical outcome, there is a need for further studies to enlighten the relation betweenH.pylorivirulence genes and antibiotic resistance.