Browsing by Author "Vural, Burcak"

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    Anti-neuronal and stress-induced-phosphoprotein 1 antibodies in neuro-Behcet's disease
    (ELSEVIER SCIENCE BV, 2011-01-01) Vural, Burcak; Ugurel, Elif; Tuzun, Erdem; Kurtuncu, Murat; Zuliani, Luigi; Cavus, Filiz; Icoz, Sema; Erdag, Ece; Gul, Ahmet; Gure, Ali O.; Vincent, Angela; Ozbek, Ugur; Eraksoy, Mefkure; Akman-Demir, Gulsen
    No disease-specific neuronal antibodies have so far been defined in neuro-Behcet's disease (NBD). Immunohistochemistry and immunocytochemistry studies showed antibodies to hippocampal and cerebellar molecular layers and the surface antigens of cultured hippocampal neurons in sera and/or cerebrospinal fluids (CSF) of 13 of 20 NBD and 6 of 20 BD patients but not in multiple sclerosis or headache controls. Screening with a protein macroarray led to identification of stress-induced-phosphoprotein-1 (STIP-1) as an antigenic target. High-titer STIP-1-antibodies were detected in 6 NBD patients' sera but not in controls. These results suggest that neuronal antibodies could be useful as diagnostic biomarkers in NBD. (C) 2011 Elsevier B.V. All rights reserved.
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    Epithelial-to-Mesenchymal Transition Is Not a Major Modulating Factor in the Cytotoxic Response to Natural Products in Cancer Cell Lines
    (MDPI, 2021-01-01) Kucukkaraduman, Baris; Cicek, Ekin Gokce; Akbar, Muhammad Waqas; Canli, Secil Demirkol; Vural, Burcak; Gure, Ali Osmay
    Numerous natural products exhibit antiproliferative activity against cancer cells by modulating various biological pathways. In this study, we investigated the potential use of eight natural compounds (apigenin, curcumin, epigallocatechin gallate, fisetin, forskolin, procyanidin B2, resveratrol, urolithin A) and two repurposed agents (fulvestrant and metformin) as chemotherapy enhancers and mesenchymal-to-epithelial (MET) inducers of cancer cells. Screening of these compounds in various colon, breast, and pancreatic cancer cell lines revealed anti-cancer activity for all compounds, with curcumin being the most effective among these in all cell lines. Although some of the natural products were able to induce MET in some cancer cell lines, the MET induction was not related to increased synergy with either 5-FU, irinotecan, gemcitabine, or gefitinib. When synergy was observed, for example with curcumin and irinotecan, this was unrelated to MET induction, as assessed by changes in E-cadherin and vimentin expression. Our results show that MET induction is compound and cell line specific, and that MET is not necessarily related to enhanced chemosensitivity.