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Permanent URI for this collectionhttps://hdl.handle.net/11443/932
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Item Chemical composition and biological activities of Valeriana dioscoridis SM roots .(ELSEVIER, 2021-01-01) Sen-Utsukarci, Burcu; Kessler, Sonja M.; Akbal-Dagistan, Ozlem; Estep, Alden S.; Tabanca, Nurhayat; Kurkcuoglu, Mine; Demirci-Kayiran, Serpil; Eroglu-Ozkan, Esra; Gul, Zulfiye; Bardakci, Hilal; Becnel, James; Kiemer, Alexandra K.; Mat, Afife; Baser, Kemal Husnu CanZika, dengue, and Yellow fever are transmitted primarily by Aedes aegypti mosquitoes. Investigations to find more natural but highly effective pesticidal agents against Ae. aegypti have increased in the last few years. Plant-based compounds have been the source of several current pesticides and have provided models for creation of synthetic derivatives like the pyrethroids. Plants continue to be a rich source for potential new active ingredients. The aim of this study is to increase knowledge on V. dioscoridis and to present the results of the cytotoxic and insecticidal activities of various extracts of its roots and volatile compound compositions of roots. The hexane and chloroform extracts, HM1 and CM1 respectively, exhibited cytotoxicity with IC50 values of 128.4 and 86.93 mg/mL against the HepG2 cell line. Because CM1 had higher cytotoxicity across the studied concentration range, it was also tested against the HUVEC cell line. Extracts HM1 and CM1 were also tested for their insecticidal activities. CM1 was found to be the more active (mortality (\%) = 96.7\% +/- 5.8 at 5 mu g/mosquito), consistent with its cytotoxic activity. Since these active extracts were nonpolar, the chemical composition of the volatile compounds of the roots were analyzed by GC-MS analysis. About 87.7\% of the essential oil were characterized. The major compound was beta-eudesmol (9.2\%), following by camphene (8.9\%), bornyl acetate (8.2\%) and maaliol (7.6\%). This is the first study on the HepG2 and HUVEC cytotoxicity and insecticidal activity of V. dioscoridis. Further studies on the potential of this plant to define the spectrum of activity and examine potential therapeutic agents is warranted. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.Item Chemical composition and antioxidant, cytotoxic, and insecticidal potential of Valeriana alliariifolia in Turkey(INST MEDICAL RESEARCH \& OCCUPATIONAL HEALTH, 2019-01-01) Sen-Utsukarci, Burcu; Taskin, Turgut; Goger, Fatih; Tabanca, Nurhayat; Estep, Alden S.; Kessler, Sonja M.; Akbal-Dagistan, Ozlem; Bardakci, Hilal; Kurkcuoglu, Mine; Becnel, James; Kiemer, Alexandra; Mat, AfifeValeriana is a common plant species used for various healing purposes in folk medicine since antiquity. This study investigates the phytochemical profile, antioxidant, cytotoxic, and insecticidal activity of Valeriana alliariifolia Adams, a species that has traditionally been used in Turkey. For the analyses we prepared four root extracts of V. alliariifolia Adams using hexane (HM1), chloroform (CM1), ethanol (EM1), and water (WM1) for maceration. Additionally, two extracts were also prepared from its roots by maceration separately with ethanol (EM2) and water (WM2). One sample was prepared as a water infusion (WI), according to the procedure used in Turkish traditional medicine. The 2,2-Diphenyl1-picrylhydrazyl (DPPH) scavenging and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation scavenging activity tests showed that ethanol extracts had the strongest antioxidant activity: EM1 (IC50 - DPPH: 17.694 mu g/mLItem Combretastatins: An Overview of Structure, Probable Mechanisms of Action and Potential Applications(MDPI, 2020-01-01) Karatoprak, Gokce Seker; Akkol, Esra Kupeli; Genc, Yasin; Bardakci, Hilal; Yucel, Cigdem; Sobarzo-Sanchez, EduardoCombretastatins are a class of closely related stilbenes (combretastatins A), dihydrostilbenes (combretastatins B), phenanthrenes (combretastatins C) and macrocyclic lactones (combretastatins D) found in the bark of Combretum caffrum (Eckl. \& Zeyh.) Kuntze, commonly known as the South African bush willow. Some of the compounds in this series have been shown to be among the most potent antitubulin agents known. Due to their structural simplicity many analogs have also been synthesized. Combretastatin A4 phosphate is the most frequently tested compounds in preclinical and clinical trials. It is a water-soluble prodrug that the body can rapidly metabolize to combretastatin A4, which exhibits anti-tumor properties. In addition, in vitro and in vivo studies on combretastatins have determined that these compounds also have antioxidant, anti-inflammatory and antimicrobial effects. Nano-based formulations of natural or synthetic active agents such as combretastatin A4 phosphate exhibit several clear advantages, including improved low water solubility, prolonged circulation, drug targeting properties, enhanced efficiency, as well as fewer side effects. In this review, a synopsis of the recent literature exploring the combretastatins, their potential effects and nanoformulations as lead compounds in clinical applications is provided.Item Herbal Ingredients in the Prevention of Breast Cancer: Comprehensive Review of Potential Molecular Targets and Role of Natural Products(HINDAWI LTD, 2022-01-01) Akkol, Esra Kuepeli; Bardakci, Hilal; Barak, Timur Hakan; Aschner, Michael; Karatoprak, Goekce Seker; Khan, Haroon; Hussain, YaseenAmong various cancers, breast cancer is the most prevalent type in women throughout the world. Breast cancer treatment is challenging due to complex nature of the etiology of disease. Cell division cycle alterations are often encountered in a variety of cancer types including breast cancer. Common treatments include chemotherapy, surgery, radiotherapy, and hormonal therapyItem Oxidative Stress and Marine Carotenoids: Application by Using Nanoformulations(MDPI, 2020-01-01) Genc, Yasin; Bardakci, Hilal; Yucel, Cigdem; Karatoprak, Gokce Seker; Akkol, Esra Kupeli; Barak, Timur Hakan; Sobarzo-Sanchez, EduardoCarotenoids are natural fat-soluble pigments synthesized by plants, algae, fungi and microorganisms. They are responsible for the coloration of different photosynthetic organisms. Although they play a role in photosynthesis, they are also present in non-photosynthetic plant tissues, fungi, and bacteria. These metabolites have mainly been used in food, cosmetics, and the pharmaceutical industry. In addition to their utilization as pigmentation, they have significant therapeutically applications, such as improving immune system and preventing neurodegenerative diseases. Primarily, they have attracted attention due to their antioxidant activity. Several statistical investigations indicated an association between the use of carotenoids in diets and a decreased incidence of cancer types, suggesting the antioxidant properties of these compounds as an important factor in the scope of the studies against oxidative stress. Unusual marine environments are associated with a great chemical diversity, resulting in novel bioactive molecules. Thus, marine organisms may represent an important source of novel biologically active substances for the development of therapeutics. Marine carotenoids (astaxanthin, fucoxanthin, beta-carotene, lutein but also the rare siphonaxanthin, sioxanthin, and myxol) have recently shown antioxidant properties in reducing oxidative stress markers. Numerous of bioactive compounds such as marine carotenoids have low stability, are poorly absorbed, and own very limited bioavailability. The new technique is nanoencapsulation, which can be used to preserve marine carotenoids and their original properties during processing, storage, improve their physiochemical properties and increase their health-promoting effects. This review aims to describe the role of marine carotenoids, their potential applications and different types of advanced nanoformulations preventing and treating oxidative stress related disorders.Item A New Perspective on the Treatment of Alzheimer's Disease and Sleep Deprivation-Related Consequences: Can Curcumin Help?(HINDAWI LTD, 2022-01-01) Kuepeli Akkol, Esra; Bardakci, Hilal; Yuecel, Cigdem; Seker Karatoprak, Goekce; Karpuz, Buesra; Khan, HaroonSleep disturbances, as well as sleep-wake rhythm disorders, are characteristic symptoms of Alzheimer's disease (AD) that may head the other clinical signs of this neurodegenerative disease. Age-related structural and physiological changes in the brain lead to changes in sleep patterns. Conditions such as AD affect the cerebral cortex, basal forebrain, locus coeruleus, and the hypothalamus, thus changing the sleep-wake cycle. Sleep disorders likewise adversely affect the course of the disease. Since the sleep quality is important for the proper functioning of the memory, impaired sleep is associated with problems in the related areas of the brain that play a key role in learning and memory functions. In addition to synthetic drugs, utilization of medicinal plants has become popular in the treatment of neurological diseases. Curcuminoids, which are in a diarylheptanoid structure, are the main components of turmeric. Amongst them, curcumin has multiple applications in treatment regimens of various diseases such as cardiovascular diseases, obesity, cancer, inflammatory diseases, and aging. Besides, curcumin has been reported to be effective in different types of neurodegenerative diseases. Scientific studies exclusively showed that curcumin leads significant improvements in the pathological process of AD. Yet, its low solubility hence low bioavailability is the main therapeutic limitation of curcumin. Although previous studies have focused different types of advanced nanoformulations of curcumin, new approaches are needed to solve the solubility problem. This review summarizes the available scientific data, as reported by the most recent studies describing the utilization of curcumin in the treatment of AD and sleep deprivation-related consequences.Item Potential Anthelmintic and Antioxidant Activities of Jasminum fruticans L. and Its Phytochemical Analysis(TABRIZ UNIV MEDICAL SCIENCES, FAC PHARMACY, 2022-01-01) Akkol, Esra Kupeli; Kozan, Esma; Bardakci, Hilal; Barak, Timur Hakan; Khalilpour, SaraBackground: Ethnobotanical investigations conducted in Turkey demonstrated that Jasminurn fruticans L. extract and fruit juice had been used against parasites in animals. In this study, the possible antihelmintic activity of various J. fruticans extracts contributing to its traditional use, was relatively assessed. In addition, the antioxidant potentials and phytochemical composition of the extracts were investigated since there is a relationship between helminthiasis, oxidative stress and phenolic metabolites. Methods: In this study, aerial parts of J. fruticans were subsequently extracted using n-hexane, ethyl acetate (EtOAc) and methanol (MeOH). In vivo anthelmintic activity of the extracts was compared with albendazole used as a reference in adult earthworms. Various methods, including free radical scavenging and metal-related activity assays, were used to assess the antioxidant capacity of the above-mentioned extracts. Assessment of phenolic composition was accomplished through total phenolic, phenolic acid, and flavonoid content assays as well as liquid chromatography-mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) scan modes. Further chlorogenic acid (3-O-caffeoylquinic acid) contents of extracts were quantified using high-performance thin-layer chromatography (HPTLC). Results: Between all tested extracts, MeOH extract at a quantity of 50.0 mg/mL, paralysed worms in 8.1 min and killed them in 12.8 min, showing a high anthelmintic effect similar to albendazole. Similarly, in vitro DPPH radical scavenging activity, cupric ion reduction and total antioxidant capacity experiments demonstrated that MeOH extract had significant antioxidant activity. Further phytochemical screening showed that. MeOH extract was richer regarding phenolic metabolites. Chlorogenic acid, ferulic acid, catfeic acid and gallic acid were only detected in the MeOH extract. Conclusion: Results justify and support the use of J. fruticans in traditional medicine as an anthelmintic agent. Furthermore, a positive correlation was found between the strong antioxidant capacity along with the phenolic composition determined in the MeOH extract and anthelmintic activity.Item Simultaneous quantification of six phenylethanoid glycosides in some Turkish Scutellaria species by a new HPLC-DAD method(TAYLOR \& FRANCIS LTD, 2019-01-01) Bardakci, Hilal; Acar, Ebru Turkoz; Kirmizibekmez, HasanA new HPLC-DAD method was developed and validated for simultaneous determination of six main phenylethanoid glycosides (calceolarioside D, neocalceolarioside D, verbascoside, isoverbascoside, leucoseptoside A and martynoside) in the aerial parts of four Scutellaria L. taxa from flora of Turkey. All standard compounds showed a good linearity (R-2 > 0.999) in a relatively wide concentration range (1-120 mu g/mL). The LOD of the compounds was in the range of 0.104-1.295 mu g/mL and the LOQ was in the range of 0.450-2.536 mu g/mL. The recoveries of the selected compounds were calculated in the range of 97.46-117.85\%. The amounts of the phenylethanoid glycosides showed variation in the extracts. The developed method was found to be accurate, precise and reproducible, and successfully applied to identify and quantify the phenylethanoid glycoside composition of Scutellaria species. {[}GRAPHICS] .Item In Vitro Anti-Aging Potential Evaluation of Maclura pomifera (Rafin.) Schneider 80\% Methanol Extract with Quantitative HPTLC Analysis(GALENOS PUBL HOUSE, 2022-01-01) Barak, Timur Hakan; Celep, Inci Kurt; Senturk, Tugba Buse; Bardakci, Hilal; Celep, EnginObjectives: Maclura pomifera (Rafin.) Schneider is a widespread species all around the world, which is also frequently cultured for ornamental purposes. Previous studies revealed that M. pomifera fruits are rich in prenylated isoflavonoids, exhibit noteworthy biological activities, and have probable benefits, particularly, when applied topically. Considering that phenolic compounds are important sources in the development of anti -aging cosmetic products, this study investigated the anti-aging potential of M. pomifera 80\% methanolic extract (MPM) by evaluating antioxidant and extracellular matrix degrading enzymes inhibiting activity. Materials and Methods: For this study, the inhibitory potential of 80\% MPM against different enzymes associated with the aging process was evaluated. Given the unequivocal role of oxidative stress in aging, in vitro antioxidant tests were employed as well. Moreover, osajin was determined as the major bioactive isoflavonoid of the sample by high performance thin layer chromatography analysis. Results: Results of the mechanistically different antioxidant assays exhibited notable antioxidant potential of the extract. Inhibition potential of MPM against hyaluronidase, collagenase, and elastase enzymes, which are directly linked to acceleration of the aging process, was investigated and results revealed that MPM inhibited the aforementioned enzymes explicitly. MPM had notable phenolic and flavonoid contentItem Effect of brewing material and various additives on polyphenolic composition and antioxidant bioactivity of commercial Tilia platyphyllos Scop. infusions(MARMARA UNIV, 2020-01-01) Bardakci, Hilal; Barak, Timur Hakan; Ozdemir, Kevser; Celep, EnginHerbal infusions have become very popular due to their pleasant flavor as well as their positive influence on health. The compositions of such infusions are affected by the extraction technique, duration, additives as well as container materials. This study implements comparison of antioxidant activities and phenolic contents of the infusions commercially purchased Tilia platyphyllos Scop. samples, one of the most preferred herbal infusions worldwide, prepared by using teapots with different materials. Antioxidant potencies of the samples were examined using tests with different mechanisms such as free radical scavenging test (DPPH), metal-related activity tests (CUPRAC, FRAP). On account of assessing the phenolic profile, total phenol, phenolic acid and flavonoid contents were estimated spectrophotometrically. In addition, the presence of protocatechuic acid in the extracts was investigated by HPTLC densitometry (between 0.762-1.037 w/w\%). Besides, antioxidant activities (DPPH, CUPRAC and TOAC) of the extracts were re-calculated after addition of natural/synthetic sweeteners, brown and white sugar, lemon, flower and pine honeys to the infusions. Results showed that the highest total antioxidant capacity was seen on Tiliae infusions prepared in ceramic teapot (672.80 +/- 1.40 mg AAE/g DE). Moreover, stevioside addition enhanced DPPH radical scavenging of Tiliae extracts (2781.76 +/- 44.38 EC50 in mu g/mL). This is the first report related with comparison of these brewing materials and additives in respect to their phenolic content and antioxidant activity of herbal teas.