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Permanent URI for this collectionhttps://hdl.handle.net/11443/932

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Author: search.filters.author.Bastu, ErcanHas files: Yes

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    Comparison of endometrial prostanoid profiles in three infertile subgroups: the missing part of receptivity?
    (ELSEVIER SCIENCE INC, 2020-01-01) Keles, Irem Demiral; Ulgen, Ege; Erkan, Melike Belkiz; Celik, Saliha Esin; Aydin, Yasemin; Onem, Ayse Nur; Kandemir, Hulya; Arslanoglu, Tugce; Apak, Mustafa Resat; Sezerman, Ugur; Yeh, John; Buyru, Faruk; Bastu, Ercan
    Objective: To study the prostanoid profile of the endometria of patients with recurrent implantation failure (RIF), unexplained infertility (UIF), and recurrent miscarriages (RM), and to compare them with the endometria of healthy fertile controls. Design: Prospective cohort study. Setting: University hospital. Patient(s): Fifteen patients with RIF, 18 patients with UIF, 16 patients with RM, and 23 fertile controls were recruited. Intervention(s): Endometrial samples were taken during the window of implantation. After tissue homogenization and extraction, analysis with ultra-performance liquid chromatography diode array detector electrospray ionisation tandemmass spectrometrywas performed. Main Outcome Measures: Concentrations of prostaglandin (PG) D1, PGE1, PGF1 alpha, 6-ketoPGF1 alpha GD2, PGE2, PGF2 alpha, 15-deoxy-Delta 12,14-PGJ2, PGD3, PGE3, PGF3 alpha, thromboxane B2, 13,14-dihydro-PGE1, 13,14-dihydro-PGF1 alpha, 13,14-dihydro-PGF2 alpha, 13,14dihydro-15-keto-PGE1, 13,14-dihydro-15-keto-PGE2, and 13,14-dihydro-15-keto-PGF2 alpha were assessed. Result(s): Comparison of the endometria of patients with UIF and the controls showed no statistically significant differences. When the endometria of patients with RIF were compared with the controls, thromboxane B2 (TXB2) was found significantly higher (843.1 pg/mg vs. 133.5 pg/mg). When the endometria of patients with RM were compared with controls, 13,14-dihydro-15-keto PGF2 alpha and TXB2 were found significantly higher (3907.30 pg/mg vs. 17.80 pg/mg and 858.7 pg/mg vs. 133.5 pg/mg respectively). Conclusion(s): We identified increased endometrial presence of TXB2 in patients with RM and RIF, and 13,14-dihydro-15-keto PGF2 alpha in patients with RM. Although common ground is observed for RM and RIF, prostanoids, on the other hand, might make their own contribution to endometrial receptivity as important as genes and proteins. Attempts to normalize the prostaglandin profile of the endometrium via enzymatic activity can open new therapeutic options. (C) 2019 by American Society for Reproductive Medicine.
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    Raman spectroscopy as a non-invasive diagnostic technique for endometriosis
    (NATURE PUBLISHING GROUP, 2019-01-01) Parlatan, Ugur; Inanc, Medine Tuna; Ozgor, Bahar Yuksel; Oral, Engin; Bastu, Ercan; Unlu, Mehmet Burcin; Basar, Gunay
    Endometriosis is a condition in which the endometrium, the layer of tissue that usually covers the inside of the uterus, grows outside the uterus. One of its severe effects is sub-fertility. The exact reason for endometriosis is still unknown and under investigation. Tracking the symptoms is not sufficient for diagnosing the disease. A successful diagnosis can only be made using laparoscopy. During the disease, the amount of some molecules (i.e., proteins, antigens) changes in the blood. Raman spectroscopy provides information about biochemicals without using dyes or external labels. In this study, Raman spectroscopy is used as a non-invasive diagnostic method for endometriosis. The Raman spectra of 94 serum samples acquired from 49 patients and 45 healthy individuals were compared for this study. Principal Component Analysis (PCA), k- Nearest Neighbors (kNN), and Support Vector Machines (SVM) were used in the analysis. According to the results (using 80 measurements for training and 14 measurements for the test set), it was found that kNN-weighted gave the best classification model with sensitivity and specificity values of 80.5\% and 89.7\%, respectively. Testing the model with unseen data yielded a sensitivity value of 100\% and a specificity value of 100\%. To the best of our knowledge, this is the first study in which Raman spectroscopy was used in combination with PCA and classification algorithms as a non-invasive method applied on blood sera for the diagnosis of endometriosis.
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    Is Endometrial Receptivity Assay (ERA) Useful in Patients with Repeated Implantation Failure Undergoing Single, Autologous Euploid Embryo Transfer?
    (IMR PRESS, 2022-01-01) Ozaltin, Selin; Celik, Hale Goksever; Takmaz, Ozguc; Yagmur, Erbil; Ozbasli, Esra; Gungor, Mete; Yeh, John; Bastu, Ercan
    Background: Our aim in this study was to evaluate whether endometrial receptivity assay (ERA) test improves single, autologous euploid frozen-thawed embryo transfer (FET) outcomes in patients with repeated implantation failure. Methods: This was a retrospective cohort study which was conducted in a University affiliated private hospital. The study included 135 patients with repeated implantation failure who underwent single, autologous euploid ERA adjusted and non-adjusted FET. Patients were stratified into three groups, patients with receptive endometrium based on the ERA test, patients with non-receptive endometrium based on the ERA test and patients who did not receive the ERA test (control group). The three groups were compared in terms of FET outcomes. Results: Of 135 patients, 73 had the ERA test results available and 62 did not have the ERA test. Of 73 patients, 28 had non-receptive endometrium and 45 had receptive endometrium. The three groups are all the same in terms of age, body mass index, type of infertility, duration of infertility, number of previously embryo transfers and infertility causes (p > 0.05). Live birth rates were 46\%, 50\% and 51\% for receptive, nonreceptive and control groups, respectively (p > 0.05). Implantation and clinical pregnancy rates were similar between the groups, as well. Conclusions: Adjusting the embryo transfer day according to the ERA test results seems to improve FET outcomes in patients with repeated implantation failure.
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    The effect of diagnostic hysteroscopy performed before fresh and frozen-thawed embryo transfer in IVF cycles on reproductive outcomes
    (GALENOS YAYINCILIK, 2021-01-01) Eserol, Fuat; Celik, Hale Goksever; Aytan, Asli Nehir; Celik, Ali; Celik, Engin; Buyru, Faruk; Bastu, Ercan
    Objective: Hysteroscopy is frequently performed in infertile women and thought to improve pregnancy rates. The data obtained from studies investigating the effect of hysteroscopy in in-vitro fertilization (IVF) cycles is variable. We aimed to evaluate the effect of hysteroscopy on pregnancy outcomes of fresh and frozen-thawed embryo transfers (FET) performed during IVF cycles. Material and Methods: The data of the 765 patients, who had IVF treatment between January 2015 and July 2017 in an infertility center, were retrospectively analyzed. Of those, 586 ( 76.6\%) patients underwent fresh embryo transfer, while 179 (23.4\%) patients underwent FET. Hysteroscopy performed by a single experienced surgeon was scheduled two months before transfer. Hysteroscopy was performed in 101/586 (17.2\%) in those undergoing fresh embryo transfer and 44/179 (24.6\%) patients in the FET group. Pregnancy outcomes of the groups were compared respectively within their own group. Results: The mean age was similar in patients in the fresh and FET groups (p=0.365, respectively). There was no difference in the number of transferred embryos between the groups ( p=0.218). In the fresh embryo group there were 246 pregnancies, of which 44 had undergone diagnostic hysteroscopy while 202 had not, (p=0.516) and 79 pregnancies in the FET group, of which 20 had undergone diagnostic hysteroscopy while 59 had not (p=0.711). There was no statistical difference according to pregnancy rate between the groups (p=0.538). Conclusion: Performing diagnostic hysteroscopy before fresh or FET does not improve the pregnancy rates.
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    Alteration of inflammatory markers and adhesion molecules in women using oral contraceptives
    (CUKUROVA UNIV, FAC MEDICINE, 2019-01-01) Ozsurmeli, Mehmet; Bastu, Ercan; Comba, Cihan; Sucu, Mete; Arslan, Erol; Misirlioglu, Selahattin; Celik, Faruk; Zeybek, Sakir Umit; Buyukoren, Ahmet
    Purpose: The aim of the study is examine the effect of oral contraceptives on the serum levels of adhesion molecules and identify if the synthetic oestrogens provide protection against atherosclerosis. Materials and Methods: The interleukin 6 (IL-6), Tumor necrosis factor alfa (TNF Alfa), Vascular cell adhesion molecule 1 (VCAM-1), Intercellular Adhesion Molecule (ICAM-1), E selectin, P selectin, C selectin, C Reactive Protein (CRP) levels and erythrocyte sedimentation rate (ESH) of patients between 18-45, without additional illness, using oral contraceptives for contraception at least for three years were compared to healthy volunteers, aged between 18-45, having regular menstruation and without any additional diseases. Results: There has been no meaningful difference between the VCAM-1, ICAM-1, E selectin, P selectin, TNF alfa, IL 6, ESH levels of the two groups. Serum CRP levels are meaningfully higher in the group using oral contraceptives. Conclusion: Oral contraceptives change the serum levels of adhesion molecules. The change in the serum CRP levels might be due to the liver first pass effect.