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Permanent URI for this collectionhttps://hdl.handle.net/11443/932

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Author: search.filters.author.Besli, Yesim

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    Evaluation of the performance of MALDI-TOF MS and DNA sequence analysis in the identification of mycobacteria species
    (TUBITAK SCIENTIFIC \& TECHNICAL RESEARCH COUNCIL TURKEY, 2018-01-01) Akyar, Isin; Cavusoglu, Cengiz; Ayas, Meltem; Surucuoglu, Suheyla; Ilki, Arzu; Kaya, Deniz Ece; Besli, Yesim
    Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100\% of MTC and in 91\% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.
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    Evaluation of patients who were followed with bacteremia in intensive care unit
    (CUKUROVA UNIV, FAC MEDICINE, 2019-01-01) Akyildiz, Ozay; Besli, Yesim; Kocagoz, Ayse Sesin
    Purpose: In this study, the microorganisms isolated from the blood cultures of the patients who are hospitalized in the general intensive care unit (ICU) of our hospital were evaluated retrospectively and it was aimed to obtain the guiding data for the appropriate empirical treatment selection. Materials and Methods: Species distribution and antimicrobial susceptibility of 163 microorganisms, isolated from blood cultures of 152 inpatients aged 18 years and older were evaluated retrospectively. Blood cultures were taken during the febrile period of inpatients who stayed in clinic were incubated in blood culture media in BACTEC automated blood culture system. Results: Of these microorganisms found, 68\% (n=111) were Gram positive bacteria, 25\% (n=40) were Gram negative bacteria, and 7\% (n=12) were fungi. The most common microorganisms were coagulase negative staphylococcus (CoNS), Escherichia coli and Candida spp. Of the Gram negative agents isolated from blood cultures, 7\% (n=12) were Escherichia coli, 6\% (n=9) were Klebsiella spp., 5\% (n=8) were Pseudomonas spp., 4\% (n=6) were Acinetobacter spp. Extended Spectrum Beta Lactamase (ESBL) was detected 75\% (n=9) of E.coli, and 78\% (n=6) of Klebsiella spp. isolate. Conclusion: Epidemiological information about species distribution and antibiotic resistance of the isolated infectious agents and will contribute to the determination of their antibiotic usage and to decrease the morbidity and mortality rates.
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    Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species
    (POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2018-01-01) Akyar, Isin; Ayas, Meltem; Karatuna, Onur; Besli, Yesim
    We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in Bacteroides spp. The minimum inhibitory concentration (MIC) for imipenem was determined with gradient test strips, and cfiA gene was investigated by polymerase chain reaction for 27 clinical Bacteroides spp. isolates. Carba NP test was performed according to recommendations of the Clinical and Laboratory Standards Institute. Among three cfiA gene harboring clinical isolates, two imipenem resistant isolates were Carba NP test positive, while the imipenem intermediate isolate was negative. Our preliminary results suggest that the Carba NP test can be useful as a rapid test to detect carbapenemases in Bacteroides species.