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Permanent URI for this collectionhttps://hdl.handle.net/11443/932

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Author: search.filters.author.Kirmizibekmez, HasanHas files: No

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    Secondary metabolites from Gentiana cruciata L. and their anti-inflammatory and analgesic activities
    (TAYLOR \& FRANCIS LTD, 2022-01-01) Konya, Rima; Reis, Rengin; Sipahi, Hande; Barta, Anita; Hohmann, Judit; Kirmizibekmez, Hasan
    A previously unreported secoiridoid glycoside, cruciatoside (1) was isolated from the aerial parts of Gentiana cruciata L. along with ten known compounds eustomoside (2), eustomorusside (3), gentiopicroside (4), 6'-O-beta-D-glucopyranosyl gentiopicroside (5), loganic acid (6), isoorientin (7), isovitexin (8), isovitexin 2''-(E)-ferulate (9), mangiferin (10), and 2-methyl-inositol (11). The chemical structures of the isolates were elucidated based on extensive 1 D and 2 D NMR experiments as well as HRMS analysis. All isolates were evaluated for their in vitro anti-inflammatory and analgesic activities. Compounds 9, 4, and 7 (200 mu M) showed moderate anti-inflammatory activity by inhibiting nitrite production from LPS-induced RAW 264.7 macrophage cells, with the inhibition rates of 39.5\%, 25.8\% and 22.9\% respectively without exhibiting substantial cytotoxicity. Besides, 1, 2, 4, and 7 exerted the highest decrease in IL-6 levels. Moreover, compound 4 showed in vitro analgesic activity by decreasing the PGE(2) level comparable to the reference drugs.
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    Simultaneous quantification of six phenylethanoid glycosides in some Turkish Scutellaria species by a new HPLC-DAD method
    (TAYLOR \& FRANCIS LTD, 2019-01-01) Bardakci, Hilal; Acar, Ebru Turkoz; Kirmizibekmez, Hasan
    A new HPLC-DAD method was developed and validated for simultaneous determination of six main phenylethanoid glycosides (calceolarioside D, neocalceolarioside D, verbascoside, isoverbascoside, leucoseptoside A and martynoside) in the aerial parts of four Scutellaria L. taxa from flora of Turkey. All standard compounds showed a good linearity (R-2 > 0.999) in a relatively wide concentration range (1-120 mu g/mL). The LOD of the compounds was in the range of 0.104-1.295 mu g/mL and the LOQ was in the range of 0.450-2.536 mu g/mL. The recoveries of the selected compounds were calculated in the range of 97.46-117.85\%. The amounts of the phenylethanoid glycosides showed variation in the extracts. The developed method was found to be accurate, precise and reproducible, and successfully applied to identify and quantify the phenylethanoid glycoside composition of Scutellaria species. {[}GRAPHICS] .
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    Simultaneous quantification of six flavonoids in four Scutellaria taxa by HPLC-DAD method
    (SOC BRASILEIRA FARMACOGNOSIA, 2019-01-01) Bardakci, Hilal; Acar, Ebru Turkoz; Kirmizibekmez, Hasan
    The objective of this study was to develop and validate a new HPLC method to quantify several flavonoids in the methanol extract prepared from the aerial parts of four Scutellaria L. taxa from flora of Turkey. A simple, sensitive and precise reversed phase HPLC-DAD method was developed and validated for simultaneous determination of six main flavonoids