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Permanent URI for this collectionhttps://hdl.handle.net/11443/932

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Author: search.filters.author.Ozen, MetehanHas files: Yes

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    Time Series Analysis of the Microbiota of Children Suffering From Acute Infectious Diarrhea and Their Recovery After Treatment
    (FRONTIERS MEDIA SA, 2018-01-01) Dinleyici, Ener C.; Martinez-Martinez, Daniel; Kara, Ates; Karbuz, Adem; Dalgic, Nazan; Metin, Ozge; Yazar, Ahmet S.; Guven, Sirin; Kurugol, Zafer; Turel, Ozden; Kucukkoc, Mehmet; Yasa, Olcay; Eren, Makbule; Ozen, Metehan; Manuel Marti, Jose; Garay, Carlos P.; Vandenplas, Yvan; Moya, Andres
    Gut microbiota is closely related to acute infectious diarrhea, one of the leading causes of mortality and morbidity in children worldwide. Understanding the dynamics of the recovery from this disease is of clinical interest. This work aims to correlate the dynamics of gut microbiota with the evolution of children who were suffering from acute infectious diarrhea caused by a rotavirus, and their recovery after the administration of a probiotic, Saccharomyces boulardii CNCM I-745. The experiment involved 10 children with acute infectious diarrhea caused by a rotavirus, and six healthy children, all aged between 3 and 4 years. The children who suffered the rotavirus infection received S. boulardii CNCM I-745 twice daily for the first 5 days of the experiment. Fecal samples were collected from each participant at 0, 3, 5, 10, and 30 days after probiotic administration. Microbial composition was characterized by 16S rRNA gene sequencing. Alpha and beta diversity were calculated, along with dynamical analysis based on Taylor's law to assess the temporal stability of the microbiota. All children infected with the rotavirus stopped having diarrhea at day 3 after the intervention. We observed low alpha diversities in the first 5 days (p-value <0.05, Wilcoxon test), larger at 10 and 30 days after probiotic treatment. Canonical correspondence analysis (CCA) showed differences in the gut microbiota of healthy children and of those who suffered from acute diarrhea in the first days (p-value <0.05, ADONIS test), but not in the last days of the experiment. Temporal variability was larger in children infected with the rotavirus than in healthy ones. In particular, Gammaproteobacteria class was found to be abundant in children with acute diarrhea. We identified the microbiota transition from a diseased state to a healthy one with time, whose characterization may lead to relevant clinical data. This work highlights the importance of using time series for the study of dysbiosis related to diarrhea.
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    Mini Epidemic of Acinetobacter Junii in a Neonatal Intensive Care Unit: Risk Factors and Control Procedures
    (GALENOS YAYINCILIK, 2015-01-01) Sandal, Gonca; Cetin, Hasan; Ozen, Metehan; Aynali, Ayse
    Aim: Acinetobacter spp. have emerged as important nosocomial pathogens in recent decades because of the increase in the incidence of antibiotic-resistant strains. Acinetobacter junii accounts for less than 1\% of isolates. Acinetobacter junii is a gram-negative, non-fermentative cocobacillus that might cause bacteremia, especially in immunocompromised patients and newborns. This article described a mini epidemic of Acinetobacter junii that has been experienced in a neonatal intensive care unit (NICU), and taken measures to control it. Materials and Methods: The outbreak occurred at Suleyman Demirel University Hospital, Pediatrics Department, Neonatal Intensive Care Unit in August 2013. The 15-bed at NICU provides care for level 3 patients. Clinical signs of sepsis were observed in 8 of 15 infants who were treated in the neonatal intensive care unit. Results: Acinetobacter junii were isolated in six infants. Isolates were from blood cultures of five patients and one was from the blood and the cerebrospinal fluid cultures of the same patient. Two preterm and five late preterm babies were affected from epidemic. A 25 week old preterm baby died because of septic shock at the fifth hour of his treatment. Conclusion: The present study confirms that Acinetobacter junii is an important nosocomial pathogen in neonatal period. As a part of rigid infection control, intravenous feedings should be prepared under aseptic conditions.
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    Mediastinal vacuum-assisted closure therapy following pediatric congenital cardiac surgery
    (BAYCINAR MEDICAL PUBL-BAYCINAR TIBBI YAYINCILIK, 2017-01-01) Aydin, Selim; Temur, Bahar; Suzan, Dilek; Kirat, Baris; Demir, Ibrahim Halil; Ozen, Metehan; Erek, Ersin
    Background: In this study, we report nine pediatric cases who developed sternal wound complications following complex congenital cardiac surgery and treated with mediastinal vacuum-assisted closure technique. Methods: Between January 2014 and December 2015, a total of 473 congenital heart operations were performed in our hospital. Of these patients, nine (8 males, 1 females