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    Evaluation of four different HPLC devices for hemoglobinopathy screening
    (WALTER DE GRUYTER GMBH, 2021-01-01) Karadag, Mujgan Ercan; Akbulut, Emis Deniz; Avci, Esin; Oguz, Esra Firat; Kader, Saadet; Abusoglu, Gulsum; Serdar, Muhittin; Yamaz, Fatma Meric
    Objective: Hemoglobinopathies are a common public health problem in Turkey. In the screening of these disorders in population, cation-exchange high performance liquid chromatography (HPLC) is accepted as the gold standard method. In this study, the aim was to assess four different HPLC devices used in hemoglobinopathy screening. Materials and methods: A total of 58 blood samples were analyzed with four different HPLC methods (Bio-Rad variant II, Agilent 1100, Tosoh G8 and Trinity Ultra2 trademarks). Results: The comparison study demonstrated a good correlation between the results of each HPLC analyzer and the reference value obtained by averaging all the HbA(2) results belonging to the methods tested in the study {[}(Tosoh G8 (r=0.988), Bio-Rad variant II (r=0.993), Agilent 1100 (r=0.98) and Trinity Ultra2 (r=0.992)]. HbA(2) determination in the presence of HbE was interfered in both BioRad variant II and Tosoh G8. Conclusion: The analyzers were found to have compatible HbA(2) results but with accompanying different degrees of proportional and systematic biases. HPLC analyzers may be affected by different hemoglobin variants at different HbA(2) concentrations, which is an important point to take into consideration during the evaluation of HbA(2) results in thalassemia screening.
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    Evaluation of optimal urine screening and confirmation cut-off values for opiates, at a national reference laboratory
    (WALTER DE GRUYTER GMBH, 2021-01-01) Karakukcu, Cigdem; Ciraci, Mehmet Zahid; Kocer, Derya; Faydali, Mine Yuce; Serdar, Muhittin Abdulkadir
    Objectives: To obtain optimal immunoassay screening and LC-MS/MS confirmation cut-offs for opiate group tests to reduce false positive (FP) and false negative (FN) rates. Methods: A total of 126 urine samples, -50 opiate screening negative, 76 positive according to the threshold of 300 ng/mL by CEDIA method - were confirmed by a full-validated in-house LC-MS/MS method. Sensitivity, specificity, FP, and FN rates were determined at cut-off concentrations of both 300 and 2,000 ng/mL formorphine and codeine, and 10 ng/mLfor heroinmetabolite 6-mono-acetyl-morphine (6-MAM). Results: All CEDIA opiate negative urine samples were negative for morphine, codeine and 6-MAM. Although sensitivity was 100\% for each cut-off
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    The assessment of autofluorescence of the crystalline lens in diabetic patients and healthy controls: can it be used as a screening test?
    (DOVE MEDICAL PRESS LTD, 2018-01-01) Pehlivanoglu, Seren; Acar, Nur; Albayrak, Sinan; Karakaya, Muharrem; Ofluoglu, Ali
    Background: Our purpose was to demonstrate if measuring lens autofluorescence (AF) with a scanning confocal biomicroscope may be used to identify subjects with undiagnosed type II diabetes mellitus (DM), and hence, for it to be used as a marker for the severity of diabetic retinopathy in diabetic patients. Patients and methods: In this cross-sectional, comparative study, lens AF was measured with scanning confocal lens fluorescence biomicroscope in diabetic and healthy groups. Full ophthalmological examination was performed. Blood tests of fasting plasma glucose, and glycosylated hemoglobin were also analyzed. The correlation between lens AF results and blood tests was evaluated in both groups. The cutoff value for the diagnosis of DM using lens AF was investigated. Results: The study included 191 subjects with a mean age of 52.09 +/- 6.75 years. One hundred and seven (56.0\%) subjects were female, and 84 (44.0\%) were male. Eighty-two (42.9\%) patients had type II DM, and 109 (57.1\%) subjects self-reported as normal. The fluorescence ratio (FR) values ranged from 0.09 to 0.46 (0.23 +/- 0.06) in the total group. Mean FR measurements of diabetic subjects were significantly higher (0.27 +/- 0.06) than those without DM (0.20 +/- 0.05), (p=0.001). A statistically significant correlation was found between glycosylated hemoglobin, fasting plasma glucose, and FR. The cutoff point for the FR according to the presence of DM was found to be 0.24 and above (p=0.001), with a sensitivity of 71.95\% and a specificity of 80.73\%. Conclusion: Measuring AF of human lens as an indirect evidence of increased advanced glycaton end products may helpful in detecting impaired glucose metabolism. Our results show highly significant correlation between possibility of DM and FR.