Araştırma Çıktıları

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Author: search.filters.author.Aksungar, Fehime Benli

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    Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism
    (PUBLIC LIBRARY SCIENCE, 2022-01-01) Agirbasli, Deniz; Kalyoncu, Minenur; Muftuoglu, Meltem; Aksungar, Fehime Benli; Agirbasli, Mehmet
    Vitamin D deficiency is common among postmenopausal women. Telomere length can be a potential protective mechanism for age-related diseases. The objective of our study is to examine the association of vitamin D supplementation on leukocyte telomere length (LTL) in healthy postmenopausal women with vitamin D deficiency. The study was designed as a placebo-controlled study to investigate the short-term effects of vitamin D supplementation and seasonal changes on vitamin D related parameters, including 25(OH)D, 1,25(OH)(2)D parathormone (PTH), Vitamin D binding protein (VDBP), vitamin D receptor (VDR), and telomere length in a cohort of postmenopausal women (n = 102). The group was divided as supplementation (n = 52) and placebo groups (n = 50). All parameters were measured before and after treatment. Serum VDBP levels were measured by ELISA method and VDR, GC (VDBP) gene expressions and relative telomere lengths were measured in peripheral blood mononuclear cells (PBMC) using a quantitative real-time PCR method. The results demonstrate that baseline levels were similar between the groups. After vitamin D supplementation 25(OH)D, 1,25(OH)(2)D, PTH and VDBP levels were changed significantly compared to the placebo group. At the end of the study period, LTL levels were significantly increased in both groups and this change was more prominent in placebo group. The change in GC expression was significant between treatment and placebo groups but VDR expression remained unchanged. Even though the study was designed to solely assess the effects of vitamin D supplementation, LTL was significantly increased in the whole study group in summer months suggesting that LTL levels are affected by sun exposure and seasonal changes rather than supplementation. The study displayed the short-term effect of Vitamin D supplementation on vitamin D, PTH levels, LTL and vitamin D associated gene expressions. The relation between Vitamin D and LTL is not linear and could be confounded by several factors such as the population differences, regional and seasonal changes in sun exposure.
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    Glutaric acidemia type II patient with thalassemia minor and novel electron transfer flavoprotein-A gene mutations: A case report and review of literature
    (BAISHIDENG PUBLISHING GROUP INC, 2018-01-01) Saral, Neslihan Yildirim; Aksungar, Fehime Benli; Aktuglu-Zeybek, Cigdem; Coskun, Julide; Demirelce, Ozlem; Serteser, Mustafa
    Glutaric acidemia type II (GA II), also known as multiple acyl-CoA dehydrogenase deficiency, is an auto-somal recessive inborn error of amino acid and fatty acid metabolism. We report a case of GA. with novel electron transfer flavoprotein (ETF)-A mutations in a 2-year-old female with thalassemia minor. The patient developed an episode of hypoglycemia and hypotonicity on the postnatal first day. Laboratory investigations revealed elevations of multiple acyl carnitines indicating glutaric acidemia type II in newborn screening analysis. Urinary organic acids were evaluated for the confirmation and revealed a high glutaric acid excretion. Genetic analysis revealed two novel mutations in the ETF-A gene, which are considered to be compound heterozygote. At the 8 mo of life ketone therapy was added, which significantly increased the neuromotor development. The patient had been closely followed for two years with carnitine, riboflavin, coenzyme Q10, and ketone supplementation in addition to a high carbohydrate diet. Although the patient had comorbidity like thalassemia minor, her neuromotor development was normal for her age and had no major health problems. This specific case expands the previously reported spectrum of this disease.
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    A rare association: celiac disease and multiple myeloma in an asymptomatic young patient
    (WALTER DE GRUYTER GMBH, 2016-01-01) Ongen, Belkiz; Aksungar, Fehime Benli; Tiftikci, Arzu; Coskun, Abdurrahman; Serteser, Mustafa; Usnsal, Ibrahim
    Celiac Disease (CD) is a gluten-sensitive enteropathy, and an autoimmune disorder involving an innate and adaptive immune response that occurs in genetically predisposed patients who are exposed to gluten-containing foods and other environmental factors. Early diagnosis and treatment are essential in preventing complications of the disease. Symptoms may appear both in childhood or adulthood by the ingestion of gluten and are usually characterized by gastrointestinal symptoms
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    Effects of peroxisome proliferator activated receptor gamma (PPAR gamma) agonist on fasting model applied neuron cultures
    (WALTER DE GRUYTER GMBH, 2022-01-01) Pinarbasi, Arzu; Pak, Meltem; Kolay, Murat; Oz Arslan, Devrim; Aksungar, Fehime Benli
    Objectives: Peroxisome proliferator activated receptor gamma (PPAR gamma) agonists used for the treatment of Diabetes Mellitus (DM), has important roles on the regulation of metabolism including ketogenesis in fasting and low glucose states. Recently PPAR gamma was proven to have antioxidant and anti-inflammatory effects on neuronal cells. Methods: In the present study, effects of pioglitazone (PPAR gamma agonist) on cell survival, energy metabolism and mitochondrial functions were investigated in glucose deprived fasting model applied SH-SY5Y (ATCC/CRL 2266) cell lines. Before and after pioglitazone treatment
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    Hepatitis C virus positive patient diagnosed after detection of atypical cryoglobulin
    (BAISHIDENG PUBLISHING GROUP INC, 2016-01-01) Ongen, Belkiz; Aksungar, Fehime Benli; Cicek, Bahattin; Akyar, Isin; Coskun, Abdurrahman; Serteser, Mustafa; Unsal, Ibrahim
    A 60-year-old male patient presented with jaundice and dark urine for three days, icteric sclerae and skin rash on his legs for six months. Laboratory inves-tigations revealed an atypical cryoglobulinemia with high hepatitis C virus (HCV)-RNA levels. Imaging studies showed cholestasis was accompanying HCV. Capillary zone electrophoresis using immunosubtraction method revealed a polyclonal immunoglobulin G and immunoglobulin A (IgA) monoclonal cryoglobulin and that IgA lambda was absent in immu-nofixation electrophoresis. After a liver biopsy, chronic hepatitis C, HCV related mixed cryoglobulinemia and cryoglobulinemic vasculitis were diagnosed and antiviral therapy was initiated. Our HCV patient presented with cryoglobulinemic symptoms with an atypical cryoglobulinemia that was detected by an alternative method: Immunosubtraction by capillary electrophoresis. Different types of cryoglobulins may therefore have a correlation with clinical symptoms and prognosis. Therefore, the accurate immunotyping of cryoglobulins with alternative methods may provide more information about cryoglobulin-generated pathology.
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    ANALYSIS OF CHANGES IN PARATHYROID HORMONE AND 25 (OH) VITAMIN D LEVELS WITH RESPECT TO AGE, GENDER AND SEASON: A DATA MINING STUDY
    (SCIENDO, 2017-01-01) Serdar, Muhittin A.; Can, Basar Batu; Kilercik, Meltem; Durer, Zeynep A.; Aksungar, Fehime Benli; Serteser, Mustafa; Coskun, Abdurrahman; Ozpinar, Aysel; Unsal, Ibrahim
    Background: 25 (OH) vitamin D3 (25(OH) D) and parathyroid hormone (PTH) are important regulators of calcium homeostasis. The aim of this study was to retrospectively determine the cut-off for sufficient 25(OH) D in a four-season region and the influence of age, seasons, and gender on serum 25(OH) D and PTH levels. Methods: Laboratory results of 9890 female and 2723 male individuals aged 38.8 +/- 22.1 years who had simultaneous measurements of 25(OH) D and PTH were retrospectively analyzed by statistical softwares. Serum 25(OH) D and PTH levels were measured by a mass spectrometry method and by an electrochemiluminescence immunoassay, respectively. Results: Mean serum 25(OH) D levels showed a sinusoidal fluctuation throughout the year and were significantly (p < 0.01) higher in summer and autumn. On the other hand, PTH levels were significantly higher (p < 0.01) in women and showed an opposite response to seasonal effects relative to 25(OH) D. Lowest levels of 25(OH) D were detected in people aged between 20 and 40 years whereas PTH hormone levels were gradually increasing in response to aging. The significant exponential inverse relationship that was found between PTH and 25(OH) D (PTH = (exp)(4.12-0.064{*}(sqrt)(25(OH) D)) (r=-0.325, R-squared=0.105, p < 0.001)) suggested that the cut-off for sufficient 25(OH) D should be 75 nmol/L. Conclusions: Our retrospective study based on large data set supports the suitability of the currently accepted clinical cut-off of 75 nmol/L for sufficient 25(OH) D. However, the issue of assessing Vitamin D deficiency remains difficult due to seasonal variations in serum 25(OH) D. Therefore, PTH measurements should complement 25(OH) D results for diagnosing Vitamin D deficiency. It is imperative that seasonally different criteria should be considered in future.