Araştırma Çıktıları

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Author: search.filters.author.Barak, Timur HakanHas files: No

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    Potential Anthelmintic and Antioxidant Activities of Jasminum fruticans L. and Its Phytochemical Analysis
    (TABRIZ UNIV MEDICAL SCIENCES, FAC PHARMACY, 2022-01-01) Akkol, Esra Kupeli; Kozan, Esma; Bardakci, Hilal; Barak, Timur Hakan; Khalilpour, Sara
    Background: Ethnobotanical investigations conducted in Turkey demonstrated that Jasminurn fruticans L. extract and fruit juice had been used against parasites in animals. In this study, the possible antihelmintic activity of various J. fruticans extracts contributing to its traditional use, was relatively assessed. In addition, the antioxidant potentials and phytochemical composition of the extracts were investigated since there is a relationship between helminthiasis, oxidative stress and phenolic metabolites. Methods: In this study, aerial parts of J. fruticans were subsequently extracted using n-hexane, ethyl acetate (EtOAc) and methanol (MeOH). In vivo anthelmintic activity of the extracts was compared with albendazole used as a reference in adult earthworms. Various methods, including free radical scavenging and metal-related activity assays, were used to assess the antioxidant capacity of the above-mentioned extracts. Assessment of phenolic composition was accomplished through total phenolic, phenolic acid, and flavonoid content assays as well as liquid chromatography-mass spectrometry (LC-MS/MS) using multiple reaction monitoring (MRM) scan modes. Further chlorogenic acid (3-O-caffeoylquinic acid) contents of extracts were quantified using high-performance thin-layer chromatography (HPTLC). Results: Between all tested extracts, MeOH extract at a quantity of 50.0 mg/mL, paralysed worms in 8.1 min and killed them in 12.8 min, showing a high anthelmintic effect similar to albendazole. Similarly, in vitro DPPH radical scavenging activity, cupric ion reduction and total antioxidant capacity experiments demonstrated that MeOH extract had significant antioxidant activity. Further phytochemical screening showed that. MeOH extract was richer regarding phenolic metabolites. Chlorogenic acid, ferulic acid, catfeic acid and gallic acid were only detected in the MeOH extract. Conclusion: Results justify and support the use of J. fruticans in traditional medicine as an anthelmintic agent. Furthermore, a positive correlation was found between the strong antioxidant capacity along with the phenolic composition determined in the MeOH extract and anthelmintic activity.
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    In Vitro Anti-Aging Potential Evaluation of Maclura pomifera (Rafin.) Schneider 80\% Methanol Extract with Quantitative HPTLC Analysis
    (GALENOS PUBL HOUSE, 2022-01-01) Barak, Timur Hakan; Celep, Inci Kurt; Senturk, Tugba Buse; Bardakci, Hilal; Celep, Engin
    Objectives: Maclura pomifera (Rafin.) Schneider is a widespread species all around the world, which is also frequently cultured for ornamental purposes. Previous studies revealed that M. pomifera fruits are rich in prenylated isoflavonoids, exhibit noteworthy biological activities, and have probable benefits, particularly, when applied topically. Considering that phenolic compounds are important sources in the development of anti -aging cosmetic products, this study investigated the anti-aging potential of M. pomifera 80\% methanolic extract (MPM) by evaluating antioxidant and extracellular matrix degrading enzymes inhibiting activity. Materials and Methods: For this study, the inhibitory potential of 80\% MPM against different enzymes associated with the aging process was evaluated. Given the unequivocal role of oxidative stress in aging, in vitro antioxidant tests were employed as well. Moreover, osajin was determined as the major bioactive isoflavonoid of the sample by high performance thin layer chromatography analysis. Results: Results of the mechanistically different antioxidant assays exhibited notable antioxidant potential of the extract. Inhibition potential of MPM against hyaluronidase, collagenase, and elastase enzymes, which are directly linked to acceleration of the aging process, was investigated and results revealed that MPM inhibited the aforementioned enzymes explicitly. MPM had notable phenolic and flavonoid content
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    Effect of brewing material and various additives on polyphenolic composition and antioxidant bioactivity of commercial Tilia platyphyllos Scop. infusions
    (MARMARA UNIV, 2020-01-01) Bardakci, Hilal; Barak, Timur Hakan; Ozdemir, Kevser; Celep, Engin
    Herbal infusions have become very popular due to their pleasant flavor as well as their positive influence on health. The compositions of such infusions are affected by the extraction technique, duration, additives as well as container materials. This study implements comparison of antioxidant activities and phenolic contents of the infusions commercially purchased Tilia platyphyllos Scop. samples, one of the most preferred herbal infusions worldwide, prepared by using teapots with different materials. Antioxidant potencies of the samples were examined using tests with different mechanisms such as free radical scavenging test (DPPH), metal-related activity tests (CUPRAC, FRAP). On account of assessing the phenolic profile, total phenol, phenolic acid and flavonoid contents were estimated spectrophotometrically. In addition, the presence of protocatechuic acid in the extracts was investigated by HPTLC densitometry (between 0.762-1.037 w/w\%). Besides, antioxidant activities (DPPH, CUPRAC and TOAC) of the extracts were re-calculated after addition of natural/synthetic sweeteners, brown and white sugar, lemon, flower and pine honeys to the infusions. Results showed that the highest total antioxidant capacity was seen on Tiliae infusions prepared in ceramic teapot (672.80 +/- 1.40 mg AAE/g DE). Moreover, stevioside addition enhanced DPPH radical scavenging of Tiliae extracts (2781.76 +/- 44.38 EC50 in mu g/mL). This is the first report related with comparison of these brewing materials and additives in respect to their phenolic content and antioxidant activity of herbal teas.