Araştırma Çıktıları
Permanent URI for this communityhttps://hdl.handle.net/11443/931
Browse
7 results
Search Results
Item Preliminary Virtual Screening Studies to Identify GRP78 Inhibitors Which May Interfere with SARS-CoV-2 Infection(MDPI, 2020-01-01) Palmeira, Andreia; Sousa, Emilia; Koseler, Aylin; Sabirli, Ramazan; Goren, Tarik; Turkcuer, Ibrahim; Kurt, Ozgur; Pinto, Madalena M.; Helena Vasconcelos, M.SARS-CoV-2 Spike protein was predicted by molecular docking to bind the host cell surface GRP78, which was suggested as a putative good molecular target to inhibit Covid-19. We aimed to confirm that GRP78 gene expression was increased in blood of SARS-CoV-2 (+) versus SARS-CoV-2 (-) pneumonia patients. In addition, we aimed to identify drugs that could be repurposed to inhibit GRP78, thus with potential anti-SARS-CoV-2 activity. Gene expression studies were performed in 10 SARS-CoV-2 (-) and 24 SARS-CoV-2 (+) pneumonia patients. A structure-based virtual screen was performed with 10,761 small molecules retrieved from DrugBank, using the GRP78 nucleotide binding domain and substrate binding domain as molecular targets. Results indicated that GRP78 mRNA levels were approximately four times higher in the blood of SARS-CoV-2 (+) versus SARS-CoV-2 (-) pneumonia patients, further suggesting that GRP78 might be a good molecular target to treat Covid-19. In addition, a total of 409 compounds were identified with potential as GRP78 inhibitors. In conclusion, we found preliminary evidence that further proposes GRP78 as a possible molecular target to treat Covid-19 and that many clinically approved drugs bind GRP78 as an off-target effect. We suggest that further work should be urgently carried out to confirm if GRP78 is indeed a good molecular target and if some of those drugs have potential to be repurposed for SARS-CoV-2 antiviral activity.Item Predictive Value of Endoplasmic Reticulum Stress Markers in Low Ejection Fractional Heart Failure(INT INST ANTICANCER RESEARCH, 2019-01-01) Sabirli, Ramazan; Koseler, Aylin; Mansur, Nesteren; Zeytunluoglu, Ali; Sabirli, Gizem Tukenmez; Turkcuer, Ibrahim; Kilic, Ismail DoguBackground/Aim: Endoplasmic reticulum (ER) stress plays a critical role in the development of cardiac hypertrophy and heart failure. Heart failure is a crucial health problem that affects 23 million people worldwide, causes approximately 2.4 million people to be hospitalized every year in the USA, and leads to the death of more than 300,000 people. In this study, we aimed to investigate the clinical significance of ER stress markers and the predictive value of acute decompensated heart failure in patients with low ejection fraction heart failure (ADHF). Patients and Methods: This is a prospective case control study. The data included laboratory parameters pertaining to patients with ADHF in the emergency service and lipid parameters obtained during their admission to the hospital. In addition, the same parameters obtained from the control group patients with chronic heart failure (CHF) during their routine polyclinic control were recorded in the data set. Admission time to the hospital and length of hospital stay were included in the data. The levels of glucose regulated protein (GRP78), protein kinase RNA-like endoplasmic reticulum kinase (PERK), and C/EBP homologous protein (CHOP) in peripheral blood serum obtained from the patients and the control group were measured using the ELISA method. Results: Serum GRP78 concentration was lower in the HF group (p=0.003) compared to the control. The median value of serum PERK concentration in the HF group was higher than that of the control group (573 pg/ml, IQR=477.5-650 vs. 495.5 pg/ml, IQR=294-648, respectively) (p=0.001). However, there were no statistically significant differences in GRP78 and PERK serum concentrations between ADHF and CHF subgroups. Receiver operating characteristic (ROC) curve analysis showed greater area under the curve (AUC) for the serum GRP78 levels of the healthy individuals (AUC=0.748, 95\% CI=0.681-0.814, p=0.0003). The serum GRP78 level was found to be 80\% sensitive and 70\% specific at 147.5 pg/ml (p=0.0003) for distinguishing healthy individuals from HF patients. In the ADHF subgroup, there was a moderate correlation between hospitalization time and serum CHOP concentrations (Spearman rho=0.586 and p=0.001). Conclusion: High GRP78 serum concentration may protect the patient from ER stress. In addition, the serum PERK level is high in patients with HF, whereas it is insufficient in predicting acute decompensation. CHOP may be useful in predicting the length of hospital stay in patients with ADHF.Item Association Between S100b Levels and COVID-19 Pneumonia: A Case Control Study(INT INST ANTICANCER RESEARCH, 2021-01-01) Mete, Ergun; Sabirli, Ramazan; Goren, Tarik; Turkcuer, Ibrahim; Kurt, Ozgur; Koseler, AylinBackground/Aim: Extracellular S100b effects are mediated by the receptor for advanced glycation end products (RAGE), which is the S100b membrane receptor. RAGE belongs to the immunoglobulin superfamily of cell surface molecules and serves as a multiligand receptor and is expressed in high abundance by alveolar type I (AT-I) cells in adult pulmonary tissue. This study aimed to provide an insight into the association between the severity of COVID19 disease and serum S100b levels during admission to the emergency department (ED). Patients and Methods: A total of 64 patients (34 mild casesItem Endoplasmic Reticulum Stress Markers in SARS-COV-2 Infection and Pneumonia: Case-Control Study(INT INST ANTICANCER RESEARCH, 2020-01-01) Koseler, Aylin; Sabirli, Ramazan; Goren, Tarik; Turkcuer, Ibrahim; Kurt, OzgurBackground/Aim: A novel human coronavirus, named SARS-COV-2, has recently caused thousands of deaths all around the world. Endoplasmic reticulum (ER) stress plays an important role in the development of diseases. Patients and Methods: We aimed to to investigate the relationship between ER stress markers in patients infected with SARS-COV-2 and patients with pneumonia. A total of 9 patients (4 patients diagnosed with pneumonia and 5 patients diagnosed with SARS-COV-2 infection) who admitted to the emergency Department with symptoms of pneumonia and SARS-COV-2 were included in the study. A total of 18 healthy individuals without any known chronic or acute disease and drug use were included as the healthy control group. Serum human glucose regulated protein 78 (GRP78), serum human C/EBP homologous protein (CHOP) and serum human phospho extracellular signal regulated kinase (PERK) levels were measured using enzyme-linked immunosorbent assay (ELISA). Results: GRP78 levels were found to be significantly higher in SARS-COV-2 positive cases compared to individuals in other groups. Serum GRP78 level median value was statistically significantly higher in SARS-COV-2-positive group compared to the other groups (p=0.0003). Serum PERK level was statistically significantly higher in SARS-COV-2-positive pneumonia cases (p=0.046). Conclusion: An association was shown between GRP78 and SARS-COV-2 infection. Although a small number of patients was investigated, these results will be important and guide future treatments of SARS-COV-2.Item High GRP78 levels in Covid-19 infection: A case-control study(PERGAMON-ELSEVIER SCIENCE LTD, 2021-01-01) Sabirli, Ramazan; Koseler, Aylin; Goren, Tarik; Turkcuer, Ibrahim; Kurt, OzgurIntroduction: Covid-19 infection was declared a global pandemic by WHO on March 11, 2020. GRP78 protein is known to be involved in the intrusion of numerous viruses. Our current study tries to provide some insight into the variation of GRP78 protein levels in patients with Covid-19 (-) pneumonia, Covid-19 (+) pneumonia, and CT negative Covid-19 infection in comparison to the normal population through a larger number of cases. Materials and methods: 42 patients who have Covid-19 (-) pneumoniaItem Work-related injuries in textile industry workers in Turkey(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2012-01-01) Serinken, Mustafa; Turkcuer, Ibrahim; Dagli, Bekir; Karcioglu, Ozgur; Zencir, Mehmet; Uyanik, EmrahBACKGROUND This study was conducted as a survey including work-related injuries (WRI) of workers in the textile and clothing industry admitted to the emergency department (ED). METHODS This prospective study included patients with WRI reportedly occurring in the textile and clothing industry over a two-year period. The study sample comprised only the casualties occurring at the workplace and while working de facto. RESULTS A total of 374 patients were eligible for the study. More than three-fourths of the study sample were females (76.2\%, n=285). A significant proportion of the patients were between 14 and 24 years of age (44.7\%, n=167). Approximately two-thirds reported that this was their first admission to a hospital related to WRI (65.8\%, n=246). WRIs occurred most frequently between 07:00-09:00 (27.3\%) and 23:00-01:00 (17.9\%). ``Carelessness{''} and ``rushing{''} were the most commonly reported causes of WRIs from the patients' perspective (40.6\% and 21.4\%, respectively). Three-fourths of the patients reported that they were using protective equipment (74.3\%, n=278). With respect to injury types, laceration/puncture/ amputation/avulsion injuries accounted for 55.6\% (n=208) of the sample. Trauma to the upper extremities was the main type of injury in 75.1\% (n=281) of the cases. CONCLUSION Broad population-based studies are needed to define the situation as a whole in WRIs in the textile and clothing industry in the country. Strict measures should be undertaken and revised accordingly to prevent WRIs in these growing sectors.Item Causes and characteristics of work-related eye injuries in western Turkey(WOLTERS KLUWER MEDKNOW PUBLICATIONS, 2013-01-01) Serinken, Mustafa; Turkcuer, Ibrahim; Cetin, Ebru Nevin; Yilmaz, Atakan; Elicabuk, Hayri; Karcioglu, OzgurObjectives: To analyze descriptive data and characteristics of work-related eye injuries (WREI) admitted into the emergency department (ED) and obtain information to utilize in planning measures to prevent WREI. Materials and Methods: This prospective study recruited patients with WREI admitted to the center in the two-year study period. Only the casualties occurred at the workplace and while working constituted the sample. The data were collected via face-to-face contact in the ED. Results: Males comprised the majority of the sample (95.3\%, n = 778) and mean age of the patients was 28.1 +/- 6.5 (range: 15-54) with the biggest percentage in between 25 and 34 years of age (46.2\%, n = 377). Most patients were working in the metal and machinery sectors (66.4\%, n = 542). Nearly half of the patients had less than 1 year of experience (50.4\%, n = 411). The most common mechanism of WREI was noted to be exposures to welding light (26.9\%, n = 219), followed by drilling/cutting injuries (21.1\%, n = 172). ``Carelessness{''} and ``hurrying up{''} were the most commonly reported causes of WREIs among `worker-related causes' (21.4\% and 16.1\%, respectively). Lack of protective measures ranked the highest among workplace-related causes (18.7\%, n = 207). Conclusions: Programs to increase awareness on workplace safety and sound preventive strategies for both parties-employers and employees are to be pursued. Occupational safety efforts should include training on workplace eye safety and campaigns to raise knowledgeability on this disease among workers.