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Permanent URI for this collectionhttps://hdl.handle.net/11443/932

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Author: search.filters.author.Vlak, Just M.Has files: Yes

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    Enhancing vector refractoriness to trypanosome infection: achievements, challenges and perspectives
    (BMC, 2018-01-01) Kariithi, Henry M.; Meki, Irene K.; Schneider, Daniela I.; De Vooght, Linda; Khamis, Fathiya M.; Geiger, Anne; Demirbas-Uzel, Guler; Vlak, Just M.; Ince, Ikbal Agah; Kelm, Sorge; Njiokou, Flobert; Wamwiri, Florence N.; Malele, Imna I.; Weiss, Brian L.; Abd-Alla, Adly M. M.
    With the absence of effective prophylactic vaccines and drugs against African trypanosomosis, control of this group of zoonotic neglected tropical diseases depends the control of the tsetse fly vector. When applied in an area-wide insect pest management approach, the sterile insect technique (SIT) is effective in eliminating single tsetse species from isolated populations. The need to enhance the effectiveness of SIT led to the concept of investigating tsetse-trypanosome interactions by a consortium of researchers in a five-year (2013-2018) Coordinated Research Project (CRP) organized by the Joint Division of FAO/IAEA. The goal of this CRP was to elucidate tsetse-symbiome-pathogen molecular interactions to improve SIT and SIT-compatible interventions for trypanosomoses control by enhancing vector refractoriness. This would allow extension of SIT into areas with potential disease transmission. This paper highlights the CRP's major achievements and discusses the science-based perspectives for successful mitigation or eradication of African trypanosomosis.
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    Comparative Analysis of Salivary Gland Proteomes of Two Glossina Species that Exhibit Differential Hytrosavirus Pathologies
    (FRONTIERS MEDIA SA, 2016-01-01) Kariithi, Henry M.; Ince, Ikbal Agah; Boeren, Sjef; Murungi, Edwin K.; Meki, Irene K.; Otieno, Everlyne A.; Nyanjom, Steven R. G.; van Oers, Monique M.; Vlak, Just M.; Abd-Alla, Adly M. M.
    Glossina pallidipes salivary gland hypertrophy virus (GpSGHV
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    Hairpin structures with conserved sequence motifs determine the 3 ` ends of non-polyadenylated invertebrate iridovirus transcripts
    (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017-01-01) Ince, Ikbal Agah; Pijlman, Gorben P.; Vlak, Just M.; van Oers, Monique M.
    Previously, we observed that the transcripts of Invertebrate iridescent virus 6 (IIV6) are not polyadenylated, in line with the absence of canonical poly(A) motifs (AATAAA) downstream of the open reading frames (ORFs) in the genome. Here, we determined the 3' ends of the transcripts of fifty-four IIV6 virion protein genes in infected Drosophila Schneider 2 (52) cells. By using ligation-based amplification of cDNA ends (LACE) it was shown that the IIV6 mRNAs often ended with a CAUUA motif. In silico analysis showed that the 3'-untranslated regions of IIV6 genes have the ability to form hairpin structures (22-56 nt in length) and that for about half of all IIV6 genes these 3' sequences contained complementary TAATG and CATTA motifs. We also show that a hairpin in the 3' flanking region with conserved sequence motifs is a conserved feature in invertebrate-infecting iridoviruses (genus Iridovirus and Chloriridovirus).