Araştırma Çıktıları

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Author: search.filters.author.Vlak, Just M.

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    Enhancing vector refractoriness to trypanosome infection: achievements, challenges and perspectives
    (BMC, 2018-01-01) Kariithi, Henry M.; Meki, Irene K.; Schneider, Daniela I.; De Vooght, Linda; Khamis, Fathiya M.; Geiger, Anne; Demirbas-Uzel, Guler; Vlak, Just M.; Ince, Ikbal Agah; Kelm, Sorge; Njiokou, Flobert; Wamwiri, Florence N.; Malele, Imna I.; Weiss, Brian L.; Abd-Alla, Adly M. M.
    With the absence of effective prophylactic vaccines and drugs against African trypanosomosis, control of this group of zoonotic neglected tropical diseases depends the control of the tsetse fly vector. When applied in an area-wide insect pest management approach, the sterile insect technique (SIT) is effective in eliminating single tsetse species from isolated populations. The need to enhance the effectiveness of SIT led to the concept of investigating tsetse-trypanosome interactions by a consortium of researchers in a five-year (2013-2018) Coordinated Research Project (CRP) organized by the Joint Division of FAO/IAEA. The goal of this CRP was to elucidate tsetse-symbiome-pathogen molecular interactions to improve SIT and SIT-compatible interventions for trypanosomoses control by enhancing vector refractoriness. This would allow extension of SIT into areas with potential disease transmission. This paper highlights the CRP's major achievements and discusses the science-based perspectives for successful mitigation or eradication of African trypanosomosis.
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    Comprehensive annotation of Glossina pallidipes salivary gland hypertrophy virus from Ethiopian tsetse flies: a proteogenomics approach
    (MICROBIOLOGY SOC, 2016-01-01) Abd-Alla, Adly M. M.; Kariithi, Henry M.; Cousserans, Franc Ois; Parker, Nicolas J.; Ince, Ikbal Agah; Scully, Erin D.; Boeren, Sjef; Geib, Scott M.; Mekonnen, Solomon; Vlak, Just M.; Parker, Andrew G.; Vreysen, Marc J. B.; Bergoin, Max
    Glossina pallidipes salivary gland hypertrophy virus (GpSGHV
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    Comparative Analysis of Salivary Gland Proteomes of Two Glossina Species that Exhibit Differential Hytrosavirus Pathologies
    (FRONTIERS MEDIA SA, 2016-01-01) Kariithi, Henry M.; Ince, Ikbal Agah; Boeren, Sjef; Murungi, Edwin K.; Meki, Irene K.; Otieno, Everlyne A.; Nyanjom, Steven R. G.; van Oers, Monique M.; Vlak, Just M.; Abd-Alla, Adly M. M.
    Glossina pallidipes salivary gland hypertrophy virus (GpSGHV
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    Temporal proteomic analysis and label-free quantification of viral proteins of an invertebrate iridovirus
    (MICROBIOLOGY SOC, 2015-01-01) Ince, Ikbal Agah; Boeren, Sjef; van Oers, Monique M.; Vlak, Just M.
    Invertebrate iridescent virus 6 (IIV-6) is a nucleocytoplasmic virus with a similar to 212 kb linear dsDNA genome that encodes 215 putative ORFs. The IIV-6 virion-associated proteins consist of at least 54 virally encoded proteins. One of our previous findings showed that most of these proteins are encoded by genes from the early transcriptional class. This indicated that these structural proteins may not only function in the formation of the virion, but also in the initial stage of viral infection. In the current study, we followed the protein expression profile of IIV-6 over time in Drosophila S2 cells by label-free quantification using a proteomic approach. A total of 95 virally encoded proteins were detected in infected cells, of which 37 were virion proteins. The expressed IIV-6 virion proteins could be categorized into three main clusters based on their expression profiles: proteins with stably low expression levels during infection, proteins with exponentially increasing expression levels during infection and proteins that were initially highly abundant, but showed slightly reduced levels after 48 h post-infection. We thus provided novel information on the kinetics of virion and infected cell-specific protein levels that assists in our understanding of gene regulation in this lesser-known DNA virus model.
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    Hairpin structures with conserved sequence motifs determine the 3 ` ends of non-polyadenylated invertebrate iridovirus transcripts
    (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2017-01-01) Ince, Ikbal Agah; Pijlman, Gorben P.; Vlak, Just M.; van Oers, Monique M.
    Previously, we observed that the transcripts of Invertebrate iridescent virus 6 (IIV6) are not polyadenylated, in line with the absence of canonical poly(A) motifs (AATAAA) downstream of the open reading frames (ORFs) in the genome. Here, we determined the 3' ends of the transcripts of fifty-four IIV6 virion protein genes in infected Drosophila Schneider 2 (52) cells. By using ligation-based amplification of cDNA ends (LACE) it was shown that the IIV6 mRNAs often ended with a CAUUA motif. In silico analysis showed that the 3'-untranslated regions of IIV6 genes have the ability to form hairpin structures (22-56 nt in length) and that for about half of all IIV6 genes these 3' sequences contained complementary TAATG and CATTA motifs. We also show that a hairpin in the 3' flanking region with conserved sequence motifs is a conserved feature in invertebrate-infecting iridoviruses (genus Iridovirus and Chloriridovirus).